This data comes from the Tianlaochi watershed sample plot. The vegetation types of the sample plot are grassland, shrub, Sabina przewalskii and Picea crassifolia. The self-made Lysimeter is mainly used to observe the soil evapotranspiration characteristics in Picea crassifolia forestry. To provide basic data for the development of watershed evapotranspiration model. At about 19:00 every day, an electronic scale with an accuracy of 1g is used to weigh the inner barrel. In case of rain, observe whether there is leakage in the leakage barrel. If there is leakage, measure the leakage amount in the leakage barrel as well. The observation period in 2011 is from May 30 to September 10. The observation period in 2012 is from June 11 to September 10. Observation instrument: 1) standard 20cm diameter rain tube rain gauge. 2) self-made lysimeter (diameter 30.5cm, barrel height 28.5). 3) Electronic balance (accuracy: 0.1g) used to observe the weight change of self-made lysimeter.

The hydrological monitoring of Picea crassifolia and main shrub vegetation types, including canopy interception, soil moisture content and stemflow, was carried out at different altitude gradients in Pailugou catchment of Qilian Mountain. The monitoring time was the dynamic monitoring of growth season in 2012 and 2013.

In the middle of July, 2011, 1. Elaeagnus angustifolia, 2. Blister. Using Li-6400 portable photosynthesis system (li-cor, USA) and li-3100 leaf area meter, the photosynthetic physiological characteristics of desert plants were observed. The symbols in the observation data have the following meanings: Obs, number of observations;Photo, net photosynthetic rate, moles of CO2 times m minus 2 times s minus 1; Cond, stomatal conductance, mol H2O•m -- 2•s -- 1;Ci, intercellular CO2 concentration, moles of CO2 times mol-1; Trmmol, transpiration rate, mmol H2O•m -- 2•s -- 1;Vpdl, water vapor pressure deficit, kPa; Area, leaf Area, cm2;Tair, atmospheric temperature, ℃; Tleaf, leaf surface temperature, ℃;CO2R, CO2 concentration in the reference chamber, moles of CO2•mol-1; CO2S, sample chamber CO2 concentration, moles of CO2•mol-1;H2OR, water in the reference chamber, mmol H2O•mol-1; H2OS, sample chamber moisture, mmol H2O•mol-1;PARo, photon flux density, mole •m -- 2•s -- 1; Rh-r, reference room air relative humidity, %;Rh-s, relative humidity of air in sample room, %; PARi, photosynthetic effective radiation, moles •m -- 2•s -- 1;Press, atmospheric pressure, kPa; Others are the state parameters of the instrument at the time of measurement.

A small lysimeter was made to simulate the natural conditions and select typical desert plants as the objects to study the water consumption of drought stress treatment. Repeat 3 times for each plant. In 2012, the soil water content was kept at (20 ± 5)% of the field water capacity, and experiments on physiological water demand and water consumption were carried out under stress. In 2013, the soil water content was kept at (10 ± 3)% of the field water capacity, and further experiments on water consumption and water consumption law were carried out under drought stress.

The leaf area of five typical species of jinjier, jilialu, jinlumei, huangxiaoba and Ganqing jinjier in Dayekou watershed of Qilian Mountain was measured by LAI-2200 canopy analyzer.

1、 Data Description: the data includes the content of silica in snowmelt water and soil water in hulugou small watershed from May 2013 to April 2014. 2、 Sampling location: the sampling point of snowmelt water is located near 600m below No.2 meteorological station, with ground elevation of 3514.45m, longitude and latitude of 99 ° 53 ′ 20.655 ″ e, 38 ° 14 ′ 14.987 ″ n. The sampling point of soil water is located at 300m above and below the No.2 meteorological station, with the longitude and latitude of 99 ° 53 ′ 31.333 ″ E and 38 ° 13 ′ 50.637 ″ n. 3、 Measurement method: the content of silica in the sample was measured by ICP-AES. Silicon dioxide is replaced by the value of Si in the solution.

1、 Data Description: the data includes the flow data of spring 02 and spring 08 in hulugou small watershed from July 10, 2014 to September 10, 2014, with the data frequency of 15 days / time. 2、 Sampling location: No.02 spring is located 30 m away from the east of the outlet of the general drainage basin, with latitude and longitude coordinates of 38 ° 16 ′ 11.44 ″ N and 99 ° 52 ′ 50.9 ″ E. Spring No. 08 is located on the side of the intersection of the East and West Branch ditches near the East Branch ditches, with latitude and longitude coordinates of 38 ° 15'27.76 "n, 99 ° 52'46.41" E.

1、 Data Description: from June 2012 to June 2013, the rainfall, river water and soil water in the basin were sampled and analyzed. 2、 Sampling location: rainfall sampling point is located in Qilian station of Chinese Academy of Sciences, with longitude and latitude of 99 ° 52 ′ 39.4 ″ e, 38 ° 15 ′ 47 ″ n; river water sampling point is located at the outlet of hulugou watershed, with longitude and latitude of 99 ° 52 ′ 47.7 ″ e, 38 ° 16 ′ 11 ″ n, with sampling frequency of once a week; soil water sampling point is located in the middle and lower part of hongnigou catchment, with sampling depth of 180cm underground and longitude and latitude of 99 ° 52 ′ 25.98 ″ E, 38 ° 15 ′ 36.11 ″ n, only one sample is taken. 3、 Test method: thermofisher TM flash 2000 and mat 253 gas stable isotope ratio mass spectrometer were used to measure the samples in 2012; l2130-i ultra-high precision liquid water and water vapor isotope analyzer was used to measure the samples in 2013.

1、 Data Description: the data includes the river temperature of the river section in No.2 catchment area of hulugou small watershed and the river section at the intersection of the East and West Branch ditches from July 2014 to September 2014. 2、 Sampling location: the coordinates of river section in No.2 catchment area are 99 ° 52 ′ 58.40 ″ e, 38 ° 14 ′ 36.85 ″ n. The cross section coordinates of the river at the junction of the East and West Branch ditches are 99 ° 52'45 "E, 38 ° 15'26.60" n.

The observation frequency is 1 time / 30 minutes with hobo automatic temperature recorder. No. 01: the observation point is located at the exit of zone III divided by Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, and the boundary point between the cold desert zone and the cold meadow zone. The coordinates of the observation point (99 ° 53 ′ 37 ″ e, 38 ° 13 ′ 34 ″ n) are 100cm from the surface of the air temperature recorder. The observation period is from July 28 to September 2, 2012. No. 02: the observation point is located at the exit of No. 2 area divided by Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, where the terrain is gentle, at the outlet of the alluvial delta valley where there is no other tributary flowing in. The observation point coordinates (99 ° 52 ′ 58 ″ e, 38 ° 14 ′ 36 ″ n) the temperature recorder in the air is 120cm from the ground surface. The observation period is from July 4, 2012 to September 6, 2012

1、 Data overview: use solinst leveloger automatic water level gauge to observe river water level, calculate flow data through water level flow curve, and manually observe the flow through self-made flow weir (see thumbnail). Due to the limited amount of manual observation data, further supplementary observation is needed to improve the water level discharge curve. 2、 Data content: we manually observe the water level and flow data of the two sections. The first section: the exit of area III divided by Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, the boundary point between cold desert zone and cold meadow zone, where the valley is deep and the bedrock is exposed. Coordinates of observation points (99 ° 53 ′ 37 ″ e, 38 ° 13 ′ 34 ″ n). The observation period is from July 21, 2012 to May 6, 2013. The observation frequency of automatic observation data is 1 time / 30 minutes from July 21 to July 25, 2012. 1 time / 15 minutes from July 25, 2012 to May 6, 2013. After September 15, 2012, there was an error in the automatic monitoring data of the observation point. The reason may be that the flow of the river channel became smaller, the probe was exposed to the air, and the water level gauge could not correctly reflect the change of the flow of the river channel. At the same time, the temperature decreased after September, and the river channel froze in winter. There was no automatic monitoring flow data during this period. The second section: the exit of No.2 area divided by Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, with flat terrain, is located at the catchment of the outlet of the alluvial delta Valley, and the south side is the shrub area. A small flow weir is built. The observation point coordinates (99 ° 52 ′ 58 ″ e, 38 ° 14 ′ 36 ″ n), and the observation frequency of automatic observation data is 1 time / 15 minutes. The observation period is from July 21, 2012 to May 6, 2013. After the observation point entered September, the river flow gradually decreased and there was no water in the river. At this time, the reading of water level gauge can not correctly reflect the change of river discharge. At the same time, our field experience shows that from September to May of the next year, the observation point is basically in a state of no water.

In this project, Ammopiptanthus mongolicus, a typical desert plant, is taken as the research object. Through optimizing the protein extraction and purification system of Ammopiptanthus mongolicus, IEF and 2-D two-dimensional electrophoresis techniques are used to obtain soluble protein electrophoresis maps of Ammopiptanthus mongolicus, and protein spots differentially expressed under drought stress are analyzed and obtained, which provides technical guarantee for subsequent mass spectrometry to identify protein functions and construct Ammopiptanthus mongolicus water stress response network.