Karyotype analysis, genomic DNA purification and genome size prediction of Ammopiptanthus mongolica, a typical desert plant in Heihe River basin (2013)

Background: this data interchange is the first data interchange of the key project of "integrated study of eco-hydrological processes in heihe basin", "genomics research on drought tolerance mechanism of typical desert plants in heihe basin".The main research targets of the key projects is a typical sand desert plants are Holly, using the current international advanced a new generation of gene sequencing technology to the whole genome sequence and gene transcription of Holly group sequence decoding, so as to explore related to drought resistance gene and gene groups, and transgenic technology in model to verify their drought resistance in plants. Process and content: as genome sequencing requires special sequencing equipment, the project is huge and the process is complex (mainly including genome library construction, sequencing, data analysis and genome assembly), so it needs to be completed by a professional sequencing company.After contacting with sequencing companies, we learned that before sequencing an unknown genome, the size and complexity of the genome should be predicted, which is a necessary prerequisite for designing sequencing schemes and strategies.Therefore, in 2013, we mainly predicted the chromosome composition, genome size and complexity of sand Holly, and successfully established the extraction and purification method of its genomic DNA.The results showed that the plant was diploid, the genome was composed of 9 staining lines (18 lines of diploid), and the genome size was 1.07G.The quality test results of the genomic DNA indicated that the requirements of the obtained DNA complex sequencing have been sent to the sequencing company for library construction and sequencing, which is now in progress.In addition, in order to obtain a large number of uniform plant materials, we have discussed the induction of callus, which has been successful.Due to these reasons, we were unable to complete the genome sequencing and submit the relevant data of sand Holly in accordance with the original plan of the project this year, mainly because we did not count the predicted contents of the genome before. Data usage: the data obtained in this year on ploidy, karyotype composition and genome size of lycopodium SPP.The success of the callus induction provides a high-quality material guarantee for the subsequent transcriptome sequencing and drought-resistance mechanism research experiments, and it is also a new contribution to the cytological and physiological research of the plant.

Interception data of precipitation for alpine shrubs in Hulu watershed (2012)

In the growing season of 2012, four typical shrub communities observed precipitation stem stream and penetrating rainfall during the experiment period.Data content: test date;Stem flow rate;Penetration rainfall, interception. Method of observation: water penetration was measured using a circular iron vessel with a diameter of 15 cm and a height of 10 cm.Since jinrumei, seabuckthorn and jinjijicinus shrub could not be observed on a single plant, after the canopy canopy density of the sample plots was determined, 9 water receivers were placed in each sample plot, so that there were water receivers under different canopy closures.This method of observing rain penetration allows for better collection of rain penetration from different parts of the underbrush.Due to the difficulty of observation and the lack of herbaceous vegetation, the interception of herbaceous under shrub was neglected.Takashima is centered on the stem, which is near the stem. One is placed at the edge of the crown and one at the middle of the crown and spoke. The Angle between each 3 containers is 120°.Six of each shrub were selected for stem flow observation.A single shrub was measured on the lower stems of all branches, and the stem flow of the trunk of the cluster shrub was measured by standard branch method, that is, the basal diameter of each branch of the selected shrub was measured.Under brush all branch stem, the use of polyethylene plastic hose cut open, card on the thickets stems directly, with a plastic adhesive tape and glass, the plastic tube directly connected to the trunk stem flow collection bottle, bottle thickness and plastic pipe, avoid rain and penetrate the rain into the collection bottle, before use after artificial experiments can precisely collect trunk stem flow.In order to reduce the error caused by evaporation in the measurement process, the penetrating rainfall and the flow of the trunk and stem were measured in time after the rain, such as the rain at night, and the samples were taken early in the morning on the second day. Data processing: the penetration rainfall is multiplied by 1.78 (conversion coefficient of different diameters of 20 cm and 15 cm) and replaced by the corresponding penetration rainfall (mm) at standard 20 cm.The measured water volume of each trunk flow collection bottle was divided by the projection area of the standard branch to obtain the trunk flow rate of the branch. The trunk flow rate of the standard branch was multiplied by the number of branches of the whole shrub to obtain the trunk flow rate of the whole shrub.According to the principle of water balance, the redistribution process of rainfall by shrub can be divided into three parts: interception, trunk flow and penetrating rainfall: IC = P - SF - TF Where, P is the rainfall outside the forest;TF is the penetrating rainfall;SF is the flow rate of the trunk.IC is the interception amount of the irrigation layer.According to the measured data of the stem flow through the rain trunk, the interception was obtained by using the above equation.