In the year of 2018, we collected the samples at Taxkorgan county, Kashgar district, the Xinjiang Uygur Autonomous Region, northwestern China. Taxkorgan county is where the Pamir Plateau located in China. Nearly all this county is on the plateau. The average elevation of this county is more the 4000 m above the sea level. And the lowest part of the county is 3100 m above the sea level. In total, 204 samples were collected in Pamir Plateau this time. The samples collected from several different species which including cattle, yak, sheep, goat, donkey, horse and chicken. Each samples have 3-4 repetitions. For each animal, we recorded the id number, species, sex, date of sample collection, GPS coordination and elevation. Also three mateched images, i.e. from head, hoof or feet and whole body, were took for each animal. All the samples taken from the animal are the blood samples. They were keep into the refrigerator until they were finally tranfered and stored in the germplasm recourses bank.
XU Feng, WANG Muyang
To analyzing the demographic history and the genetic mechanism underlying local adaptation of the domestic Equus animals in Qinghai-Tibet Plateau and surrounding regions and building a genetic resources bank of Equus in Pan-Third Pole, we resequenced 236 domestic Equus animal samples collected until 2018, including Tibet horse, Tibet ass, domestic horses and donkeys in the plains. By applying mitochondrial DNA sequencing and D-loop sequencing on 75 samples, including 73 ass and two horses, , a batch of genetic and genome data were generated. It provides basic genetic data to analysis on domestication, immigration and expansion of domestic animals in Qinghai-Tibet Plateau. Meanwhile it helps better understand the adaption of domestic Equus animal to Qinghai-Tibet Plateau environment.
LI Yan
Cryptosporidium spp. and E. bieneusi were tested by nested PCR on domestic animals (405 fecal samples from yaks, Tibetan sheep, camels and horses, etc.) in the areas covered by the qinghai-tibet plateau mainly in Tibet and qinghai.1. The overall infection rate of cryptosporidium was 2.96% (12/405), and the detection rate of camels, Tibetan sheep and yaks in qinghai was divided into 15%, 9.8% and 3.1%.The detection rate of yaks in yunnan was 3.1%.No other domestic animals were found in Tibet or yunnan.Two cryptosporidium subspecies were detected in qinghai camels, among which c. ovis subtype was the first detected in camels.C.ryanae subtype was first detected in yaks in yunnan.The overall detection rate of E. bieneusi in domestic animals in qinghai-tibet plateau was 19.75% (80/405), and a total of 9 known subtypes and a new subtype (YN) were detected.The highest detection rate was for camels (45%) in qinghai, followed by Mongolian sheep (42.1%), yak (37.5%), horse (15.62%) and Tibetan sheep (7.3%).The detection rate of Tibetan sheep in Tibet was 10.8%.The detection rates of goats and cattle in yunnan were 36% and 25.7% respectively.CAM2 subtype was first detected in qinghai horses and CAM1 subtype was first detected in yaks.A new subtype YN was detected in yunnan cattle.
ZHANG Zhichao, DUAN Ziyuan
In order to describe the distribution pattern of genetic diversity of main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas (Pan third pole area), and clarify its related genetic background. In 2020, we extracted the total DNA from 201 global chicken blood, tissue and other DNA tissue samples, built a database and sequenced the whole genome. At the same time, we downloaded the published chicken genome data, and carried out population analysis of 863 chicken genomes, so as to provide basic data for exploring the historical events of domestication, migration and expansion of domestic chickens in the pan third pole region, and further explore the adaptation mechanism of domesticated animals to harsh environments such as drying. Articles related to this data set have been published. All data in this data set can be downloaded online from fastq, BAM, VCF and SNP files.
PENG Minsheng
Ochotona curzoniae is a small herbivorous animal peculiar to the Qinghai Tibet Plateau, which mainly inhabits the open alpine meadow, grassland and desert grassland with an altitude of 2800-5000 meters. In this sub project (2019QZKK05010212), plateau pika, a small constant temperature mammal that is extremely sensitive to environmental changes, is proposed to be selected as the representative to compare the differences in morphology, physiology and life history of pika populations at different altitudes on the Qinghai Tibet Plateau and adjacent areas through field surveys. This data set includes individual photos, habitat photos and work photos taken in Qinghai in 2020 and Maduo County, Tibet Autonomous Region in 2021, including more than 10 photos of plateau pika caves and one pika activity video.
ZHANG Xueying
Plateau pika is a key species of the Qinghai Tibet Plateau and an indigenous species formed with the uplift of the Qinghai Tibet Plateau. During the long-term evolution, it has evolved a unique life history strategy to adapt to the extreme environment of the plateau. This sub project (2019QZKK05010410) investigates the distribution area of plateau pika, analyzes its population fluctuation rule and its influencing factors in the context of global climate change, and discusses the ecological significance of plateau pika in the alpine meadow ecosystem. This data set contains the information table of 213 plateau zokor tissue samples collected in Gonghe County, Guinan County, Hainan Prefecture, and Maqin County, Golog Prefecture, Qinghai Province in 2020, including species, collection place, collection time, collection person, sample type and other information. The information table is named after the sub subject number - year - group and opened in excel
QU Jiapeng
In order to find out the current resource quantity, distribution and utilization status of Tibetan yaks and lay a foundation for the conservation and utilization of the diversity of Tibetan yaks, this sub project (2019QZKK05010705) will investigate the genetic resources of yaks and collect tissue samples in Tibet Autonomous Region from 2021-2022, including Chawula yaks (20), Jiangda yaks (21), Uqi like yaks (65), Pali yaks (20), Sibu yaks (20) Tibetan alpine yaks (20 heads). This data set includes 6 tissue sample information tables, photos and videos. The information table records information such as gender, age, weight, body height, sampling place, etc. The photos include individual appearance photos, habitat photos, and work videos.
XIN Jinwei
In order to complete the investigation of Tibetan sheep resources on the Qinghai Tibet Plateau and its surrounding areas and master the current situation of Tibetan sheep resources, the investigation of Tibetan sheep germplasm resources will be carried out in Maqu County and Xiahe County of Gannan Tibetan Autonomous Prefecture in Gansu Province in 2020, and 500 blood and tissue samples will be collected. This data set contains a tissue sample information table, including species, species, collection place, collection time, sample type and other information, which is stored in excel format. Take 100 individual photos, 10 habitat photos, 9 work photos and 2 videos. Photos are stored in jpg format and videos are stored in mp4 format. 50000 genotype data are generated for each individual, and the SNP genome typing data of 500 individuals in total are stored in "ped" and "map" formats.
LI Menghua
In order to complete the investigation of Tibetan sheep resources on the Qinghai Tibet Plateau and its surrounding areas and master the current situation of Tibetan sheep resources, the investigation of Tibetan sheep germplasm resources will be carried out in Qinghai, Gansu, Qinghai, Guizhou, Shaanxi, Yunnan, Xinjiang and Sichuan from 2021-2022, and 1021 blood and tissue samples will be collected. This data set contains a tissue sample information table, including species, species, collection place, collection time, sample type and other information, which is stored in excel format. 230 individual photos, 61 habitat photos, 22 work photos and 6 videos were taken. Photos are stored in jpg format and videos are stored in mp4 format. 50000 genotype data are generated for each individual, and the SNP genome typing data of 1000 individuals in total are stored in "ped" and "map" formats.
LI Menghua
In order to describe the distribution pattern of genetic diversity of important livestock and poultry germplasm resources in the Qinghai Tibet Plateau, clarify their related genetic background, and establish a corresponding genetic resource bank. In 2022, the survey of genetic resources of domestic animals will be carried out in Jiulong County, Hongyuan County and Xiangcheng County of Sichuan Province, and 484 blood and tissue samples of sheep, yaks, goats, dogs, pigs and cattle will be collected, 40 sheep feces samples, 2 RNA samples of Tibetan chickens and 3 RNA samples of Tibetan pigs will be collected. This data set includes 1 sample information table and 685 individual photos, 12 work photos, 5 habitat photos and 12 work videos. The sample information table contains basic sample information such as species, varieties, detailed sampling places, sample types, collection time, collectors, and storage methods, which are stored in excel form. Photos are stored in jpg format and videos are stored in MP4 format.
PENG Minsheng
In order to describe the distribution pattern of genetic diversity of important livestock and poultry germplasm resources in the Qinghai Tibet Plateau, clarify their related genetic background, and establish a corresponding genetic resource bank. During 2019-2022, 2167, 1056 and 516 tissue samples of local Tibetan sheep and fine wool sheep were collected in Gangca County, Haibei Tibetan Autonomous Prefecture, Qinghai Province, and 2074 and 1548 lambing records were recorded. This data set includes 3 tissue sample information tables and 2 lambing record information tables. The organization sample information table records the variety, collection place, collection time, sample type and other information. The lambing record information table records the variety, detailed sampling place, sex, date of birth, birth weight and other information. The information table is stored in excel form.
ZHAO Kai
This data includes bacterial 16S ribosomal RNA gene sequence data from 25 lakes in the middle of the Qinghai Tibet Plateau. The sample was collected from July to August 2015, and the surface water was sampled three times with a 2.5 liter sampler. The samples were immediately taken back to the Ecological Laboratory of the Beijing Qinghai Tibet Plateau Research Institute, and the salinity gradient of the salt lake was 0.14~118.07 g/L. This data is the result of amplification sequencing. Concentrate the lake water to 0.22 at 0.6 atm filtration pressure μ The 16S rRNA gene fragment amplification primers were 515F (5 '- GTGCCAAGCCGCGGTAA-3') and 909r (5 '- GGACTACHVGGGTWTCTAAT-3'). The Illumina MiSeq PE250 sequencer was used for end-to-end sequencing. The original data was analyzed by Mothur software. The sequence was compared with the Silva128 database and divided into operation classification units (OTUs) with 97% homology. This data can be used to analyze the microbial diversity of lakes in the Qinghai Tibet Plateau.
KONG Weidong
This data includes the distribution data of soil bacteria in Namco region of the Qinghai Tibet Plateau, which can be used to explore the seasonal impact of fencing and grazing on soil microorganisms in Namco region. The sample was collected from May to September 2015, and the soil samples were stored in ice bags and transported back to the Ecological Laboratory of Beijing Institute of Qinghai Tibet Plateau Research; This data is the result of amplification sequencing, using MoBio Powersoil ™ Soil DNA was extracted with DNA isolation kit, and the primers were 515F (5 '- GTGCCAAGCGCCGGTAA-3') and 806R (5'GGACTACNVGGGTWTCTAAT-3 '). The amplified fragments were sequenced by Illumina Miseq PE250. The original data is analyzed by Qiime software, and then the similarity between sequences is calculated, and the sequences with a similarity of more than 97% are clustered into an OTU. The Greengenes reference library is used for sequence alignment to remove the sequence that only appears once in the database. The soil moisture content and soil temperature were measured by a soil hygrometer, and the soil pH was measured by a pH meter (Sartorius PB-10, Germany). The soil nitrate nitrogen (NO3 −) and ammonium nitrogen (NH4+) concentrations were extracted with 2 M KCl (soil/solution, 1:5), and analyzed with a Smartchem200 discrete automatic analyzer. This data set is of great significance to the study of soil microbial diversity in arid and semi-arid grasslands.
KONG Weidong
Data on soil bacterial diversity of grassland in Qinghai Tibet Plateau. The samples were collected from July to August 2017, including 120 samples of alpine meadow, typical grassland and desert grassland. The soil surface samples were collected and stored in ice bags, and then transported back to the ecological laboratory of the Beijing Qinghai Tibet Plateau Research Institute. The soil DNA was extracted by MO BIO PowerSoil DNA kit. The 16S rRNA gene fragment amplification primers were 515F (5 '- GTGCCAAGCCGGTAA-3') and 806R (5 ´ GGACTACNVGGGTWTCTAAT-3 ´). The amplified fragments were sequenced by Illumina Miseq PE250. The original data is analyzed by Qiime software, and the sequence classification is based on the Silva128 database. Sequences with a similarity of more than 97% are clustered into an operation classification unit (OTU). This data systematically compares the bacterial diversity of soil microorganisms in the Qinghai Tibet Plateau transect, which is of great significance to the study of the distribution of microorganisms in the Qinghai Tibet Plateau.
KONG Weidong
The data set of bacterial post-treatment products and conventional water quality parameters of some lakes in the third pole in 2015 collected the bacterial analysis results and conventional water quality parameters of some lakes in the Qinghai Tibet Plateau during 2015. Through sorting, summarizing and summarizing, the bacterial post-treatment products of some lakes in the third pole in 2015 are obtained. The data format is excel, which is convenient for users to view. The samples were collected by Mr. Ji mukan from July 1 to July 15, 2015, including 28 Lakes (bamuco, baimanamuco, bangoso (Salt Lake), Bangong Cuo, bengcuo, bieruozhao, cuo'e (Shenza), cuo'e (Naqu), dawaco, dangqiong Cuo, dangjayong Cuo, Dongcuo, eyaco, gongzhucuo, guogencuo, jiarehbu Cuo, mabongyong Cuo, Namuco, Nier CuO (Salt Lake), Norma Cuo, Peng yancuo (Salt Lake), Peng Cuo, gun Yong Cuo, Se lincuo, Wu rucuo, Wu Ma Cuo, Zha RI Nan Mu Cuo, Zha Xi CuO), a total of 138 samples. The extraction method of bacterial DNA in lake water is as follows: the lake water is filtered onto a 0.45 membrane, and then DNA is extracted by Mo bio powerOil DNA kit. The 16S rRNA gene fragment amplification primers were 515f (5'-gtgccagcmgcgcggtaa-3') and 909r (5'-ggactachvggtwtctaat-3'). The sequencing method was Illumina miseq PE250. The original data were analyzed by mothur software, including quality filtering and chimera removal. The sequence classification was based on the silva109 database. The archaeal, eukaryotic and unknown source sequences had been removed. OTU classifies with 97% similarity and then removes sequences that appear only once in the database. Conventional water quality detection parameters include dissolved oxygen, conductivity, total dissolved solids, salinity, redox potential, nonvolatile organic carbon, total nitrogen, etc. The dissolved oxygen is determined by electrode polarography; Conductivity meter is used for conductivity; Salinity is measured by a salinity meter; TDS tester is used for total dissolved solids; ORP online analyzer was used for redox potential; TOC analyzer is used for non-volatile organic carbon; The water quality parameters of total nitrogen were obtained by Spectrophotometry for reference.
YE Aizhong
The original TIFF files of figure 1-100 of the monograph 'The Batrisini of Tibet: unveiling an enigmatic ant-loving beetle diversity at Earth’s “Third Pole” (Coleoptera, Staphylinidae, Pselaphinae)' are uploaded.
YIN Ziwei
In order to study the population evolution history and local adaptive genetic mechanism of the main domesticated equine animals in the Qinghai Tibet Plateau and its surrounding areas, and to establish the corresponding germplasm genetic resource bank. We sequenced the equine samples collected in Qinghai Province, Tibet Autonomous Region and Xinjiang Autonomous Region from the Qinghai Tibet Plateau and surrounding areas, including Tibetan donkey, plain donkey and other breeds. Sequencing includes denove and resequencing data, which provides data for tracing the historical events of domestication, migration, expansion and other groups of the main equine domesticated animals in the region, and further exploring the adaptation mechanism of equine animals to the harsh environment such as hypoxia, cold, and dryness. At the same time, all tissues of domestic donkeys were sequenced, including hifi genome data and HIC genome data, to prepare for the assembly of complete donkey genome and facilitate subsequent analysis.
LI Yan
Through the scientific research work in 2019 and 2020, the second Tibetan Plateau Scientific Expedition and Research Task 5 Theme 3 Topic 4 Lichen Scientific Research Team (2019QZKK050304) has supplemented the collection of a large number of lichen collection gaps in the Tibetan Plateau region. 2019 scientific research conducted in-depth lichen biodiversity examination for the first time in the Ali region in northern Tibet, and in 2020, fieldwork and specimen collection will be conducted in the lichen collection gap areas of Hoh Xil and Sanjiangyuan. These expeditions have unveiled the mystery of lichen composition in the Tibetan Plateau region and filled the gaps in the domestic collection of this region. This dataset contains information on 10,283 lichen specimens collected from July 2019 to September 2020 in Tibet Autonomous Region, Qinghai Province, Sichuan Province, and Yunnan Province, including information on collection habitat, collection time, collector, latitude and longitude, altitude, and Latin scientific name. Contains 4,328 specimen photos, including lichen specimen No. 815 in 2019 with 2,425 photos and specimen No. 543 in 2020 with 1,903 photos. The physical specimens are stored in the Herbarium, Kunming Institute of Botany, CAS (KUN). Specimen collection information and field ecological photographs are synchronized between various databases, including the Biotracks database and the KUN herbarium database, to facilitate later research, collation and query by relevant personnel. The specimens are now sorted by time, region and genus name and stored separately in the KUN herbarium to facilitate subsequent studies, and the corresponding molecular materials are preserved or molecular sequences are obtained, laying a good material basis for subsequent taxonomic and systematics studies of the specimens. DNA extraction and systematic taxonomic studies of various groups are also being carried out.
WANG Xinyu
The dataset contains microbial amplicon sequencing data from a total of 269 ice samples collected from 15 glaciers on the Tibetan Plateau from November 2016 to August 2020, including 24K Glacier (24K), Dongkemadi Glacier (DKMD), Dunde Glacier (DD), Jiemayangzong Glacier (JMYZ), Kuoqionggangri Glacier (KQGR), Laigu Glacier (LG), Palung 4 Glacier (PL4), Qiangtang 1 Glacier (QT), Qiangyong Glacier (QY), Quma Glacier (QM), Tanggula Glacier (TGL), Xiagangjiang Glacier (XGJ), Yala Glacier (YA), Zepugou Glacier (ZPG), ZhufengDongrongbu Glacier (ZF). The sampling areas ranged in latitude and longitude from 28.020°N to 38.100°N and 86.28°E to 95.651°E. The 16s rRNA gene was amplified by polymerase chain reaction (PCR) using 515F/907R (or 515F/806R) primers and sequenced with the Illumina Hiseq2500 sequencing platform to obtain raw data. The selected primer sequences were "515F_GTGYCAGCMGCCGCGGTAA; 907R_CCGTCAATTCMTTTRAGTTT" "515F_GTGCCAGCMGCCGCGG; 806R_ GGACTACHVGGGTWTCTAAT". The uploaded data include: sample number, sample description, sampling time, latitude and longitude coordinates, sample type, sequencing target, sequencing fragment, sequencing primer, sequencing platform, data format and other basic information. The sequencing data are stored in sequence file data format forward *.1.fq.gz and reverse *.2.fq.gz compressed files.
LIU Yongqin
From October to November 2021, we used camera traps to collect mammal diversity and distributions along the elevational gradients at the Yarlung Zangbo Grand Canyon National Nature Reserve. We retrived images of 262 camera traps placed between May 2021 and October 2021. We obtained 12391 pictures of wild animals,41 species of large and medium mammals were recorded.. The camera traps were reset in the same locations after renew batteries and memory cards.Camera trap data could inventory endangered species in the region, and provide information to identify biodiversity hotspots and conservation priorities.
LI Xueyou
In order to describe the distribution pattern of genetic diversity of main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas (Pan third pole area), and clarify its related genetic background. In 2020, we extracted the total DNA from 266 global chicken blood, tissue and other DNA tissue samples, built a database and sequenced the whole genome. At the same time, we downloaded the published chicken genome data, and carried out population analysis of 863 chicken genomes, so as to provide basic data for exploring the historical events of domestication, migration and expansion of domestic chickens in the pan third pole region, and further explore the adaptation mechanism of domesticated animals to harsh environments such as drying. Articles related to this data set have been published. All data in this data set can be downloaded online from fastq, BAM, VCF and SNP files.
PENG Minsheng
We investigated and collected the germplasm resources of cyanine in the Qinghai Tibet Plateau and its surrounding areas, carried out homogenous garden experiments to obtain phenotypic data, used genome sequencing technology to obtain data libraries and construct high-quality reference genomes. Using the re sequencing technology to analyze the structure of the cyanine population, combined with the early human migration and diffusion routes, this paper explores the historical process of the formation of the modern geographical distribution pattern of the cyanine on the Qinghai Tibet Plateau. By correlation analysis with phenotypic data, the adaptive mechanism of modern populations of cyanine was analyzed. Understand the environmental differences of the pan third pole and the impact of human activities and cultural differences in different regions on the migration, adaptation and domestication of plants on the Qinghai Tibet Plateau from the whole genome level.
DUAN Yuanwen
We investigated and collected the germplasm resources of cyanine in the Qinghai Tibet Plateau and its surrounding areas, carried out homogenous garden experiments to obtain phenotypic data, used genome sequencing technology to obtain data libraries and construct high-quality reference genomes. Using the re sequencing technology to analyze the structure of the cyanine population, combined with the early human migration and diffusion routes, this paper explores the historical process of the formation of the modern geographical distribution pattern of the cyanine on the Qinghai Tibet Plateau. By correlation analysis with phenotypic data, the adaptive mechanism of modern populations of cyanine was analyzed. Understand the environmental differences of the pan third pole and the impact of human activities and cultural differences in different regions on the migration, adaptation and domestication of plants on the Qinghai Tibet Plateau from the whole genome level.
DUAN Yuanwen
We investigated and collected the germplasm resources of cyanine in the Qinghai Tibet Plateau and its surrounding areas, carried out homogenous garden experiments to obtain phenotypic data, used genome sequencing technology to obtain data libraries and construct high-quality reference genomes. Using the re sequencing technology to analyze the structure of the cyanine population, combined with the early human migration and diffusion routes, this paper explores the historical process of the formation of the modern geographical distribution pattern of the cyanine on the Qinghai Tibet Plateau. By correlation analysis with phenotypic data, the adaptive mechanism of modern populations of cyanine was analyzed. Understand the environmental differences of the pan third pole and the impact of human activities and cultural differences in different regions on the migration, adaptation and domestication of plants on the Qinghai Tibet Plateau from the whole genome level.
DUAN Yuanwen
The Second Tibetan Plateau Scientific Expedition and Research Task V Theme III "Conservation and Sustainable Utilization of Plateau Microbial Diversity" (2019QZKK0503) carried out more than 30 field scientific expeditions in the first and second years. Footprints cover most of the Tibetan Plateau, including the investigation of glaciers (such as Qiangyong Glacier, Tanggula Glacier, Everest East Rongbu glacier, Jiemayangzong Glacier, Palung 4 Glacier, etc.), lakes, soils, fungi, lichens, animals in Southeast Tibet, Qiangtang Plateau, Cocosili and Himalayan region. The dataset contains 6,471 photos and videos, including habitat photos, working photos, and scientific images collected during the first and second years of fieldwork.
LIU Yongqin
In 2021， the birds along elevation gradients in Gangrigabu Mountains were investigated by point count method. With a 400-meter elevational gradient, elevation zones were set up in the survey area. Five elevation zones were built in the north slope from TongMai Town to Galong Temple in Bome County, and 8 elevation zones were built in the south slope from Jiefang Bridge to Galongla in Medog County. So that we can make clear about the pattern and maintenance mechanism of bird diversity along elevation gradients in this region. The data of bird diversity and distribution will be used to further explore the key scientific issues such as the impact of climate change on bird diversity and adaptation strategies, and the response and protection strategies of bird species diversity under the global climate change.
WANG Jie
Hoh Xil area is located in the hinterland of Qinghai Tibet Plateau, south of Kunlun Mountain, north of Tanggula Mountain and west of Qinghai Tibet highway. From May to August 1990, the comprehensive scientific investigation team of Kekexili region, jointly organized by the State Science and Technology Commission, the Chinese Academy of Sciences, the Qinghai provincial government and the State Environmental Protection Bureau, with the strong support of the Qinghai provincial government, carried out a large-scale comprehensive investigation in the region and obtained rich first-hand precious information. Biologists all over the world have realized more and more clearly that the most urgent task at present is to conduct a comprehensive investigation of some species before they are stable or extinct in the natural ecosystem, and the investigation of species diversity is the most critical, because if we do not correctly understand the species in a certain area and their role in the natural ecosystem, it will be seriously damaged Affect the development of other work. Most of the Hoh Xil area in Qinghai Province is still a no man's land. There is almost no investigation and Research on the biodiversity and resources in this area. Therefore, this scientific research is of great scientific significance.
FENG Zuojian
The data of birds in Kekexili area of Qinghai Province are from the comprehensive scientific investigation of Kekexili area carried out from May to August 1990. In the composition of birds, all of them are Palaearctic, and the composition of Qinghai Tibet area accounts for a large proportion, reaching as many as 18 species. Kekexili is closely related to the whole plateau in terms of geological structure or physical and geographical conditions, and its bird composition also reflects The correlation is analyzed. On the one hand, the composition of the Qinghai Tibet region accounts for a large proportion; on the other hand, the local habitat formed by its special location is extremely arid, which makes it difficult for the composition of the Qinghai Tibet region to extend into its hinterland; on the other hand, the composition of the Inner Mongolia new area is difficult to adapt due to the high temperature and cold, which makes the bird species, distribution and population in the Qinghai Tibet Plateau extremely low Different from other areas of the plateau, Hoh Xil area has always been an uninhabited area, lacking zoological data, which is conducive to the study of Hoh Xil area.
FENG Zuojian
Plateau medicine and human physiology research in Kekexili area of Qinghai Province, the data are from the comprehensive scientific investigation of Kekexili area carried out from May to August 1990. With the human marching into the plateau area, plateau medicine, a main branch of environmental medicine, is quietly rising. Although the research of plateau medicine has gone through more than a century (1987), many mechanisms remain unresolved. Medical scientists have organized academic mountaineering for many times to carry out Alpine medical investigation (West JB, 1982; Wu T, y, 1992). However, most of them are limited to observing the changes of the internal environment of the aircraft through the intense movement of climbing mountains, which is far from the daily life and labor of human beings on the plateau. After almost all the mountains in the world have been conquered by human beings, there are still a few highland hinterlands that have not been set foot in. Hoh Xil is such a "no man's land" on the earth. The magical color and adventurous career are attracting brave explorers. The effects of high altitude hypoxia, low temperature, low humidity and strong ultraviolet on human body are the most attractive research fields in Applied Physiology.
FENG Zuojian
The data of insects and other arthropods in Kekexili area of Qinghai Province are from the comprehensive scientific investigation of Kekexili area from May to August 1990. The central area of the Qinghai Tibet Plateau, known as the "roof of the world", is Kekexili area. Due to its high terrain, sparse vegetation, low air temperature and less rainfall, its insect fauna is extremely unique, which is a Typical Alpine insect fauna. Because of the high altitude, cold climate, low air pressure, lack of fresh water, no roads and extremely bad natural conditions in Hoh Xil area. From May 1990 to August 1990, more than 4000 specimens were collected, including ten orders of Insecta: Collembola, Ephemeroptera, zhenptera, trichophaga, Hemiptera, Coleoptera, Lepidoptera, Diptera, Hymenoptera and fleas, and three other classes of invertebrates, such as Branchiopoda and labiopoda Chilopoda and Arachnida. In Insecta, Diptera is the most, followed by Coleoptera and Hymenoptera. It provides a reference for the following research.
FENG Zuojian
Certain hydrothermal conditions and soil conditions are the basis of crop growth. The Tibetan Plateau covers a vast area, and the changes of altitude and surface form in different areas are extremely complex. Plateau climate and soil have obvious spatial changes, so the distribution of cultivated land and crops has a large regional. There are two groups of high mountain ranges running east-west and north-south in Tibet, forming the basic framework of the plateau. The vast plateau is distributed between the mountains, and there are many low mountains, hills, lake basins and valley inlaid in the meantime, the overall terrain of the whole region gradually picked up from the southeast to the northwest, the southeast is lower, the west, the north is higher. The majestic Himalayas stand on the southern and western borders of China and India, China and Nepal, China and Tin, China and Bhutan, and China and Pakistan. With a total length of 2,400 kilometers and a mountain width of 200-300 kilometers, and an average elevation of more than 6,000 meters, they constitute a natural barrier to the southern part of the Qinghai-Tibet Plateau. The warm and wet airflow in the Bay of Bengal is blocked by mountains. The climate on the southern slope of the Himalayas is warm and humid, while that on the northern foothills of the Himalayas is warm and cool and dry, forming two different climate regions on the southern and northern slopes. The south side of the Himalayas mountain rivers deep, mountain canyon landform. The valley is more than 3000 meters above sea level, and the climate is warm and humid. The soil types are rich, mainly including mountain yellow soil, mountain brown soil, mountain brown soil and mountain meadow soil, etc. The soil is acidic to neutral, and contains rich humus, high nitrogen content, coarse texture and good permeability. Abundant surface runoff, irrigation conditions are better. However, due to terrain limitations, most of the land cannot be used because the slope is greater than 25 degrees, and most of the land is covered by forests. Arable land is mainly distributed in the valley below 4000 meters, the area is very limited.
LU Jimei
Naked barley, called highland barley in Tibet, is the main crop of the Tibetan plateau and the main food of the Tibetan people. It has a long planting history, the largest planting area, the widest distribution and the highest planting ceiling. As for the autonomous region, the annual sown area and total grain output account for more than half of the total sown area and total grain output. In river valleys below 4,200 meters, they account for 30-50% of the area sown to local crops. In the alpine farming area of 4,200-4,500 meters above sea level, it accounts for 70-90% of the planting area of local crops. Naked barley is almost the only crop at agricultural sites above 4,500 meters. Tibetan bare barley has strong cold and drought resistance and ADAPTS to the plateau climate conditions. It is distributed in the vast agricultural areas and farming-pastoral interlacing areas from more than 1,000 meters to 4,500 meters above sea level. At present, the upper limit of planting reaches 4750 meters, which is the highest limit of crop distribution in Tibet. In 1974, a comprehensive scientific expedition to the Qinghai-Tibet Plateau entered Tibet from Sichuan province and carried out investigations along the route. During a survey of wild barley in Jitang district, Chaya County, Qamdo region, Tibetan members provided information about semi-wild wheat, which is similar to wheat in morphology but has broken cob at maturity, and is generally mixed with highland barley and wheat fields as a field weed. Plant specimens and seeds were collected. When the expedition team arrived at the Institute of Agricultural Science and Technology of Tibet Autonomous Region, they learned that comrades Cheng Tianqing and Dong Yuao had collected a kind of wheat with broken cob at maturity in SAN 'an Qu Lin, Longzi County, Shannan Prefecture in 1962, which belonged to the same type as the materials we had collected in other areas of Tibet. Later, in the Xigaze regional agricultural research institute, Comrade Tan Changhua said that he had also seen this kind of wheat when the cob broke at maturity in Renbu County. In addition to the above areas, this wild wheat was also found near Jiacha county, Gongga County and Longzi County in Shannan during this year's investigation. Currently known distribution areas include lancang River basin, Yarlung Zangbo River basin and Longzi River basin.
LU Jimei
Agriculture in Tibet is concentrated in the valleys of the Yarlung Zangbo River in south Tibet and the Nu, Lancang and Jinsha Rivers in east Tibet. The agricultural area of the valley accounts for 75 percent of the total cultivated land area of the autonomous region, and the grain output accounts for more than 80 percent of the total grain output of the autonomous region. Wheat and naked barley (known as highland barley in Tibet) are the main grain crops in The Tibet Autonomous Region, with the perennial sown area accounting for more than 80% of the total sown area, while the wheat and naked barley in the valley agricultural areas account for 75% of the sown area and 82% of the total yield of the region. The agricultural area of the valley is located between 28 ° and 31° north latitude, 2700 -- 4100 meters above sea level. It belongs to the temperate climate of the plateau, with better soil and water conservancy conditions and higher crop yield. But before liberation, under the dark feudal serfdom, the people lived in extreme poverty, and agricultural production was very backward, with the yield of grain per mu only over 100 jin. After liberation, especially since 1972, winter wheat was widely promoted in the agricultural areas of the valley, which promoted the reform of the farming system and significantly increased grain output. In 1975, the total grain output of the region increased by more than 50 percent compared with 1965, and by more than 1.5 times that of 1958 before the democratic reform. In 1977, the area sown with winter wheat was nearly 700,000 mu, accounting for about 20 percent of the grain sown area. The planting area of winter wheat has expanded from areas with an altitude of less than 3,000 meters to areas with an altitude of less than 4,100 meters, and the Tibetan Plateau has developed from a historical area of spring wheat into an area where both spring and winter wheat are grown. In 1977, the average yield of winter wheat per mu in the agricultural areas of the valley exceeded 400 jin, and that of bare barley and spring wheat also reached 300 jin per mu.
LU Jimei
The animal resources and their reasonable protection, development and utilization in Hoh Xil area of Qinghai Province are based on the comprehensive scientific investigation of Hoh Xil area carried out from May to August 1990. With the rapid increase of population in this century and the increasing intensity of human demand for natural resources, the resources have been seriously damaged, and the deterioration of natural environment and the increasing depletion of resources have become the focus of the world One of the most prominent problems in the world. In particular, the living environment of wild animals is still in a completely natural state, which is rare on earth. Most of Hoh Xil area is still a no man's land. It retains the original state of the ecosystem, and is also a refuge for many rare and unique resource animals. Therefore, it is a rare treasure land with rich animal resources in China and the world. Because of the bad natural conditions and the extremely difficult transportation in Hoh Xil area, zoologists have not been involved in this area for a long time. From May to August 1990, the animal group of the national comprehensive scientific investigation team of Hoh Xil region had the honor to go deep into the region to carry out resource investigation. They had a relatively clear understanding of the resource background and mastered a large number of first-hand materials, thus filling the scientific blank of animal resources in this region.
FENG Zuojian
People in the plain often have altitude reaction after entering Tibet. In order to deeply analyze the change pattern of genomic expression profile in the process of altitude response and altitude acclimation. Based on the mRNA transcriptome sequencing method, we have obtained the transcriptome sequencing data of 46 individuals before entering Tibet (collection place: Chongqing). We first collected the peripheral blood samples of 46 plain Han individuals, treated the peripheral blood with red blood cell lysate (Tiangen), centrifuged at 4000 rpm for 10 min, separated and extracted white blood cells, and extracted the total RNA of each sample by Trizol method. Then 46 libraries were constructed by poly (a) capture method. Poly (a) + mRNA library was isolated from 1 g total RNA of each sample with oligo (DT) beads. The construction of RNA SEQ library was carried out according to the preparation scheme of truseq RNA library. The 46 RNA libraries were double ended sequenced using novaseq platform. The sequencing results were 150 BP reads fastq files, and the data volume of each sample exceeded 6.0 GBP. The transcriptome data of plain people before entering Tibet can be used as the baseline data after entering Tibet. By comparing and analyzing the transcriptome data of plain people before and after entering Tibet, screening the significantly differentially expressed genes before and after entering Tibet, and annotating the biological functions of differentially expressed genes, we can deeply analyze the gene expression change mode and function regulation network mechanism in the process of altitude reaction and altitude acclimatization.
KONG Qingpeng
In order to collect the special germplasm resources of Qinghai Tibet Plateau and excavate the molecular markers affecting the special germplasm resources, individual sheep with excellent ectopic spots were selected for marker assisted selection, propagation and generation breeding according to the genetic marker information, so as to cultivate the families of special germplasm resources. In 2021, this sub project (2019QZKK05010704) widely collected samples of Qinghai Tibetan sheep and Qinghai fine wool sheep in Haibei, Qinghai, and continued to establish and expand the first and second core groups in Ledu agricultural experimental station and Qinghai Sanjiaocheng sheep breeding farm. This data set contains the basic information of 1050 tissue samples, including variety, collection place, collection time, gender, tissue type, preservation method, etc.
ZHAO Kai
In order to analyze the genomic characteristics, domestication, population history, genetic diversity and population differentiation characteristics of large forehead cattle, as well as the genetic basis of excellent meat economic traits of large forehead cattle, this sub project (2019QZKK05010703) investigated the genetic resources of Dulong cattle and Gaofeng cattle in Kunming and Wenshan Miao Autonomous Prefecture of Yunnan Province in 2021, and collected tissue samples of heart, liver, spleen, lung and kidney. Provide scientific theoretical reference for the protection, development and utilization of large cattle germplasm resources, and promote the development of high-end beef cattle industry in China. This data set contains individual photos and tissue sample information table, which records basic information such as variety, collection place, collection time, sample type, sampling position and so on.
LI Yan
In order to analyze the animal diversity pattern of the Qinghai Tibet Plateau and establish the corresponding animal specimen database. In 2021, this sub project (2019QZKK05010109) focused on Liangshan Yi Autonomous Prefecture and Ganzi Tibetan Autonomous Prefecture. A total of 271 specimens of local wild animals, such as Apodemus agrarius and social rats, were collected. The solid samples include animal individuals, skins, tissues, etc. This data set contains one specimen information table (271 copies), one tissue sample information table (271 copies), and photos taken by Tibet Chayu moto infrared camera. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel. Photos, stored in JPG format.
LI Xueyou
In order to describe the distribution pattern of genetic diversity of important rodents in the Qinghai Tibet Plateau, clarify their related genetic background, and establish the corresponding genetic resource bank. In 2021, this sub project (2019QZKK05010410) focused on the investigation of plateau pika in Haixi Mongolian and Tibetan Autonomous Prefecture, Golog Prefecture and Hainan prefecture, Qinghai. A total of 200 plateau pika samples were collected, and the solid samples were spleen and lung tissues. This data set contains a sample information table and habitat photos, work photos and work videos. The sample information table contains basic sample information such as species, gender, detailed sampling place, altitude, sample type, collection time, collector and storage method, which are stored in the form of Excel.
QU Jiapeng
The dataset includes pictures of culturable bacterial colonies from Amuco (AMC), Bangkog Co (BGC), Palung 4 glacier (PL4) and Yuzhufeng (YZF) ice core on the Tibetan Plateau, which can reflect basic information such as color and transparency of colonies. A table of information is attached, including the latitude and longitude of the colony's origin, water depth and color, etc. In this experiment, different bacteria were isolated from ice, snow, lake and other habitats on the Qinghai Tibet Plateau. These bacteria show different colors, which may be related to the bacteria adapting to the environment of strong radiation, low temperature and oligotrophic on the Qinghai Tibet Plateau, and adapting to the harsh environment through different pigment production capacity. The colonies are obtained by coating the lake water plate and culturing at low temperature (4 ℃) (R2A medium, etc.) to determine that they are pollution-free and single colonies. 16S rRNA full-length sequencing will be carried out for the obtained single colony, and whether it is a new species will be determined by 97% similarity; If the sequence similarity between this strain and the most similar strain is less than 97%, a whole genome mapping will be carried out to determine the phylogenetic relationship of this strain in the corresponding genera, families and even phyla, and determine whether this strain has a new developmental status to supplement the microbial diversity of lakes on the Tibetan Plateau.
LIU Yongqin
Using satellite tracking technology, this sub project (2019QZKK05010409) tracked the overwintering population in the middle of black necked Crane. After catching the black necked Crane by the foot button method, we adopt the foot ring method to fix the tracker, which greatly improves the tracking achievement rate and the amount of data obtained. When the power is ideal, the sampling frequency can be accelerated to one point in half an hour. Get more detailed tracking data. The relevant results show that the selection of wintering sites for black necked cranes is highly stable. Preliminary analysis shows that there is a strong connecting line between wintering sites and breeding sites, but there are also the characteristics of larvae in different summers. The strong migration connectivity indicates that black necked cranes are more vulnerable to environmental changes. This data set contains 200 habitat photos of black necked cranes and 1276 activity data of young black necked cranes. The satellite tracking data sheet of young black necked Crane recorded longitude and latitude, date, altitude, speed, temperature and other information.
WU Heqi
This sub project (2019qzkk05010411) focuses on the population investigation and monitoring of wild yak, Tibetan antelope and brown bear, carries out systematic and continuous field investigation, finds out their geographical distribution, population number and population structure, and carries out appropriate habitat assessment. The distribution area and potential distribution area are selected and divided into multiple sub areas. Stratified random sampling is adopted according to habitat type, climate, altitude and other factors, and the population counting method, variable distance spline method or fixed width spline method are used for investigation respectively. At the same time, in view of the extremely low encounter rate of brown bears, combined with the interview method. The investigation area mainly includes the distribution areas and potential distribution areas of the three animals in Yushu Tibetan Autonomous Prefecture, Haixi Mongolian Tibetan Autonomous Prefecture, Haibei Tibetan Autonomous Prefecture and other prefectures and counties in Qinghai Province, in order to master the distribution and five-year population dynamics of wild yak, Tibetan antelope and brown bear in Qinghai Province. This data set contains photos of yaks, Tibetan antelopes and brown bears investigated in Qinghai in 2021.
CHEN Zhenning
Information of animal samples, tissue samples, DNA bar code samples and other physical samples collected in the second year (from the end of 2020 to 2021) of the fifth topic of the second comprehensive scientific investigation and research task of the Qinghai Tibet Plateau - "conservation and sustainable utilization of animal diversity on the plateau" (2019QZKK0501). All data shall be sorted according to the subject and sub subject, and the folder shall be named according to the subject number and sub subject number. Each sample table contains one or more sample information tables. Each information table contains sub topic number, species, collection place, collection time, collector, sample type, storage method and other information.
ANIMAL RESOURCE PLATFORM OF QINGHAI-TIBET PLATEAU Animal Resource Platform of Qinghai-Tibet Plateau
In order to describe the distribution pattern of genetic diversity of pika germplasm resources in Qinghai Tibet Plateau, clarify its related genetic background, and establish the corresponding genetic resource bank. In 2021, this sub project (2019QZKK05010209) focused on Qinghai Province (Haixi Mongolian and Tibetan Autonomous Prefecture, Golmud City, Kunlun mountain pass; Haixi Mongolian and Tibetan Autonomous Prefecture, Dulan County, Xiangride Town, Gouli township; Golog Tibetan Autonomous Prefecture, Maduo County; Golog Tibetan Autonomous Prefecture, Maqin County, Dawu town; Huangnan Tibetan Autonomous Prefecture, Zeku County; Hainan Tibetan Autonomous Prefecture, Guinan County, Taxiu township) 93 plateau pika germplasm resources were collected at different altitudes, and the solid samples included blood or tissue, feces and so on. This data set contains 1 sample information table. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel.
ZHANG Liangzhi
In order to describe the distribution pattern of genetic diversity of important rodents on the Qinghai Tibet Plateau, clarify their relevant genetic background, analyze their population fluctuation law and influencing factors under the background of global climate change, analyze the impact of plateau pika on birds and its significance in maintaining ecosystem stability, and to explore the adaptation and evolution mechanism of plateau species and build a population outbreak prediction and early warning system, It is of great theoretical and practical significance to correctly understand the status and role of plateau pika in ecosystem and put forward timely and effective population management countermeasures. This sub project (2019qzkk05010410) focuses on the investigation of plateau pika in Haixi Mongolian and Tibetan Autonomous Prefecture, Golog Prefecture and Hainan prefecture of Qinghai in 2021. A total of 200 tissue samples such as spleen and lung of plateau pika were collected. This data set contains 1 sample information table. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel.
QU Jiapeng
Plateau Zokor and plateau pika are endemic and key species widely distributed in the Qinghai Tibet Plateau and surrounding plain areas. Different geographical locations and ecological environment have led to diversified species formation. The research on the origin and evolution of these species needs to be improved. At the same time, their geographical distribution and morphological characteristics are good materials for studying plateau adaptation and adaptive evolution. Comparing the analysis of genome, transcriptome and epigenetics between Plateau and other low altitude plain related species can help to analyze the mechanism of plateau adaptation and preliminarily explore the mechanism of apparent modification in plateau adaptation. In 2021, a total of 112 tissue samples of 16 animals were collected in Qinghai Province. This data set contains a sample information table, which contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, and is stored in the form of Excel. In order to explore the plateau adaptive evolution of flagship species on the plateau, chip SEQ sequencing and transcriptome sequencing were carried out on 16 individuals of Plateau Zokor and Pika in Maduo County and Laji mountain, Qinghai Province. Each individual produced 15GB data, a total of 600gb. The data is stored in fastq format.
LV Xuemei
In order to describe the effects of environmental changes and human disturbance on the temporal and spatial distribution of small mammals in the Qinghai Tibet Plateau, statistical models and molecular techniques were used to identify the species of small mammals with different altitude gradients. In 2021, 105 small mammal resources such as plateau pika, Meriones meridionalis, three toed jerboa, five toed jerboa and Qinghai squirrel were collected in 8 areas of Xihai Town, Gangcha county and Delingha city of Qinghai Province. The solid samples include animal solid samples and muscles, heart, liver, spleen, lung, kidney, pancreas, bladder, testis and ovary. This data set contains one specimen information table, one tissue sample information table and one photo corresponding to each specimen. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel. Photos, stored in JPG format.
HOU Xiang
In order to determine the distribution points and habitat types of plateau forest frog along the latitudinal gradient in Qinghai, in 2021, a total of 8 distribution points of plateau forest frog were collected in Minhe County of Haidong, Qinghai, Gonghe County of Hainan Tibetan Autonomous Prefecture and Maqin County of Golog Prefecture, covering an altitude of 2000-3800m. This dataset contains 1 coordinate information table and 57 habitat photos. The coordinate information table contains information such as number, recording date, time, weather, coordinate longitude and latitude, altitude sample, habitat type and photo number of representative habitat, which are stored in the form of Excel. Photos are stored in JPG format. In order to reveal the impact of climate change on the diversity of plateau forest frogs on the Qinghai Tibet Plateau in the future, the thermal safety margin of different geographical populations of plateau forest frogs was calculated through the data of thermophysiological indexes and environmental effective temperature, and the threat of climate warming of different geographical populations of plateau forest frogs was evaluated. This data set includes field activity body temperature, resting metabolic rate, ambient temperature and morphological data of four altitude populations of plateau forest frog, which supplements the selected body temperature, tolerance temperature and temperature correction data of 2000 m altitude populations. The data is stored in Excel format. In order to study the differences of genetic diversity of plateau forest frog at different altitudes, 100 samples of plateau forest frog collected from four altitudes (2000 m, 2600 m, 3200 m and 3800 m) of Qinghai Tibet Plateau were sequenced and analyzed based on the sequences of four mitochondrial genes (12S rRNA, 16S rRNA, coi and cytb), so as to provide scientific basis for the protection of this species. This data includes 12S rRNA, 16S rRNA The sequence data obtained from the sequencing of COI and cytb genes supplement the sequence data of four genes of the population at an altitude of 2000m. The data is stored in FASTA format
ZHANG Yongpu
In order to analyze the phenotype and genetic adaptation of different populations of sand lizard in Tibet to environmental changes, this sub project (2019QZKK05010216) selects the vertical zone of altitude gradient in the plateau as the main line, collects samples throughout the distribution area of red tailed sand lizard, compares the differences of morphology, physiology and life history of different populations, and analyzes their phenotype and genetic adaptation to environmental changes, Combined with the species distribution model and mechanism model, the threatened degree of red tailed sand lizard in the future is predicted, and the Protection Countermeasures of plateau sand lizard under the background of climate warming are put forward, which provides a theoretical basis for the protection of Reptile Diversity on the Qinghai Tibet Plateau under the background of environmental change. In 2021, sand lizard sample resources were collected in Jiuquan, Gansu Province and Delingha, Qinghai Province. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel. Photos, stored in JPG format.
JI Xiang
This sub project (2019QZKK05010217) plans to select Qinghai sand lizard, an exothermic vertebrate that is very sensitive to environmental changes, as the representative. Through field investigation, we will compare the differences in morphology, physiology and life history of Qinghai sand lizard populations at different altitudes in the Qinghai Tibet Plateau and adjacent areas, and analyze the response and adaptation characteristics of Qinghai sand lizard to environmental changes in the Qinghai Tibet Plateau, Combined with species distribution model (SDM) and mechanism model, this paper predicts the threatened degree of Qinghai sand lizard in the future, and puts forward the Protection Countermeasures of Qinghai sand lizard diversity on the Qinghai Tibet Plateau under the background of climate warming, so as to provide a theoretical basis for the protection of Reptile Diversity on the Qinghai Tibet Plateau under the background of environmental change. This data includes ecological photos and habitat photos of Qinghai sand lizard in Gonghe County, Haiyan County and Maduo County of Qinghai Province.
DU Weiguo
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