Background: this data interchange is the first data interchange of the key project of "integrated study of eco-hydrological processes in heihe basin", "genomics research on drought tolerance mechanism of typical desert plants in heihe basin".The main research targets of the key projects is a typical sand desert plants are Holly, using the current international advanced a new generation of gene sequencing technology to the whole genome sequence and gene transcription of Holly group sequence decoding, so as to explore related to drought resistance gene and gene groups, and transgenic technology in model to verify their drought resistance in plants. Process and content: as genome sequencing requires special sequencing equipment, the project is huge and the process is complex (mainly including genome library construction, sequencing, data analysis and genome assembly), so it needs to be completed by a professional sequencing company.After contacting with sequencing companies, we learned that before sequencing an unknown genome, the size and complexity of the genome should be predicted, which is a necessary prerequisite for designing sequencing schemes and strategies.Therefore, in 2013, we mainly predicted the chromosome composition, genome size and complexity of sand Holly, and successfully established the extraction and purification method of its genomic DNA.The results showed that the plant was diploid, the genome was composed of 9 staining lines (18 lines of diploid), and the genome size was 1.07G.The quality test results of the genomic DNA indicated that the requirements of the obtained DNA complex sequencing have been sent to the sequencing company for library construction and sequencing, which is now in progress.In addition, in order to obtain a large number of uniform plant materials, we have discussed the induction of callus, which has been successful.Due to these reasons, we were unable to complete the genome sequencing and submit the relevant data of sand Holly in accordance with the original plan of the project this year, mainly because we did not count the predicted contents of the genome before. Data usage: the data obtained in this year on ploidy, karyotype composition and genome size of lycopodium SPP.The success of the callus induction provides a high-quality material guarantee for the subsequent transcriptome sequencing and drought-resistance mechanism research experiments, and it is also a new contribution to the cytological and physiological research of the plant.
HE Junxian, GU Lifei
1. Data overview The data set of the base camp integrated environmental observation system is a set of ENVIS (IMKO, Germany) which was installed at the base camp observation point by qilian station.It is stored automatically by ENVIS data mining system. 2. Data content This data set is the scale data from January 1, 2012 to December 31, 2012.Including air temperature 1.5m, humidity 1.5m, air temperature 2.5m, humidity 2.5m, soil moisture 0cm, precipitation, wind speed 1.5m, wind speed 2.5m, wind direction 1.5m, geothermal flux 5cm, total radiation, surface temperature, ground temperature 20cm, ground temperature 40cm, ground temperature 60cm, ground temperature 80cm, ground temperature 120cm, ground temperature 160cm, CO2, air pressure. 3. Space and time scope Geographical coordinates: longitude: 99° 53’e;Latitude: 38°16 'N;Height: 2980.2 m
SI Jianhua
1. Data overview The data set of the base camp integrated environmental observation system is a set of ENVIS (IMKO, Germany) which was installed at the base camp observation point by qilian station.It is stored automatically by ENVIS data mining system. 2. Data content This data set is the scale data from January 1, 2012 to December 31, 2012.Including air temperature 1.5m, humidity 1.5m, air temperature 2.5m, humidity 2.5m, soil moisture 0cm, precipitation, wind speed 1.5m, wind speed 2.5m, wind direction 1.5m, geothermal flux 5cm, total radiation, surface temperature, ground temperature 20cm, ground temperature 40cm, ground temperature 60cm, ground temperature 80cm, ground temperature 120cm, ground temperature 160cm, CO2, air pressure. 3. Space and time scope Geographical coordinates: longitude: 99° 53’e;Latitude: 38°16 'N;Height: 2980.2 m
JIA Li
In mid-july 2011, photosynthetic organs (leaves or assimilating branches) of typical desert plants were collected and brought back to the laboratory in a liquid nitrogen tank for determination. The analysis indexes mainly include soluble protein unit: mg/g;Free amino acid unit: g/g;Chlorophyll content unit: mg/g;Superoxide dismutase (SOD) unit: U/g FW;Catalase (CAT) unit: U/(g•min);POD unit: U/(g•min);Proline (Pro) unit: g/g; Soluble sugar unit: g/g;Malondialdehyde (MDA) is given in moles per liter.
SU Peixi
1. Data overview The data set of the base camp integrated environmental observation system is a set of ENVIS (IMKO, Germany) which was installed at the base camp observation point by qilian station.It is stored automatically by ENVIS data mining system. 2. Data content This data set is the scale data from January 1, 2011 to December 31, 2011.It mainly includes two layers of temperature, humidity and wind, six layers of soil water content, precipitation, 5cm geothermal flux, total radiation, seven layers of soil temperature, CO2 and air pressure. 3. Space and time scope Geographical coordinates: longitude: 99° 53’e;Latitude: 38°16 'N;Height: 2980.2 m
CHEN Rensheng, HAN Chuntan
On the basis of physiological and biochemical analysis of photosynthetic organs (leaves or assimilating branches) of typical desert plants in heihe river basin collected in mid-july 2011, some photosynthetic organs of desert plants were collected in mid-july 2012 and put into a liquid nitrogen tank and brought back to the laboratory for determination. Physiological analysis indexes mainly include: soluble protein unit: mg/g;Free amino acid unit: g/g;Chlorophyll content unit: mg/g;Superoxide dismutase (SOD) unit: U/g FW;Catalase (CAT) unit: U/(g•min);POD unit: U/(g•min);Proline (Pro) unit: g/g; Soluble sugar unit: g/g;Malondialdehyde (MDA) is given in moles per liter.
SU Peixi
1. Data overview: This data set is the scale meteorological gradient data of qilian station from January 1, 2012 to December 31, 2012 (installed at the end of September 2011).VG1000 gradient observation system carries out long-term monitoring of wind speed, wind direction, air temperature, humidity, radiation and other conventional meteorological elements, and carries out data storage and processing analysis in combination with the data collector with high precision and high scanning frequency. 2. Data content: The main observation factors include four layers of air temperature, humidity and two-dimensional ultrasonic wind, rain and snow volume meter, eight layers of ground temperature, soil moisture content, etc. 3. Space and time range: Geographical coordinates: longitude: longitude: 99° 52’e;Latitude: 38°15 'N;Height: 3232.3 m
CHEN Rensheng, HAN Chuntan
1. Data overview: This data set is the scale meteorological gradient data of qilian station from January 1, 2013 to December 31, 2013 (installed at the end of September 2011).VG1000 gradient observation system carries out long-term monitoring of wind speed, wind direction, air temperature, humidity, radiation and other conventional meteorological elements, and carries out data storage and processing analysis in combination with the data collector with high precision and high scanning frequency. 2. Data content: The main observation factors include four layers of air temperature, humidity and two-dimensional ultrasonic wind, rain and snow volume meter, eight layers of ground temperature, soil moisture content, etc. 3. Space and time range: Geographical coordinates: longitude: longitude: 99° 52’e;Latitude: 38°15 'N;Height: 3232.3 m
CHEN Rensheng, HAN Chuntan
In the growing season of 2012, four typical shrub communities observed precipitation stem stream and penetrating rainfall during the experiment period.Data content: test date;Stem flow rate;Penetration rainfall, interception. Method of observation: water penetration was measured using a circular iron vessel with a diameter of 15 cm and a height of 10 cm.Since jinrumei, seabuckthorn and jinjijicinus shrub could not be observed on a single plant, after the canopy canopy density of the sample plots was determined, 9 water receivers were placed in each sample plot, so that there were water receivers under different canopy closures.This method of observing rain penetration allows for better collection of rain penetration from different parts of the underbrush.Due to the difficulty of observation and the lack of herbaceous vegetation, the interception of herbaceous under shrub was neglected.Takashima is centered on the stem, which is near the stem. One is placed at the edge of the crown and one at the middle of the crown and spoke. The Angle between each 3 containers is 120°.Six of each shrub were selected for stem flow observation.A single shrub was measured on the lower stems of all branches, and the stem flow of the trunk of the cluster shrub was measured by standard branch method, that is, the basal diameter of each branch of the selected shrub was measured.Under brush all branch stem, the use of polyethylene plastic hose cut open, card on the thickets stems directly, with a plastic adhesive tape and glass, the plastic tube directly connected to the trunk stem flow collection bottle, bottle thickness and plastic pipe, avoid rain and penetrate the rain into the collection bottle, before use after artificial experiments can precisely collect trunk stem flow.In order to reduce the error caused by evaporation in the measurement process, the penetrating rainfall and the flow of the trunk and stem were measured in time after the rain, such as the rain at night, and the samples were taken early in the morning on the second day. Data processing: the penetration rainfall is multiplied by 1.78 (conversion coefficient of different diameters of 20 cm and 15 cm) and replaced by the corresponding penetration rainfall (mm) at standard 20 cm.The measured water volume of each trunk flow collection bottle was divided by the projection area of the standard branch to obtain the trunk flow rate of the branch. The trunk flow rate of the standard branch was multiplied by the number of branches of the whole shrub to obtain the trunk flow rate of the whole shrub.According to the principle of water balance, the redistribution process of rainfall by shrub can be divided into three parts: interception, trunk flow and penetrating rainfall: IC = P - SF - TF Where, P is the rainfall outside the forest;TF is the penetrating rainfall;SF is the flow rate of the trunk.IC is the interception amount of the irrigation layer.According to the measured data of the stem flow through the rain trunk, the interception was obtained by using the above equation.
SONG Yaoxuan, LIU Zhangwen
"Hydrological ecological economic process coupling and evolution of Heihe River Basin Management under the framework of water rights" (91125018) project data collection 2 - Dunhuang comprehensive plan for rational utilization of water resources and ecological protection (2011-2020) Planning documents mainly include: 1. Current situation and existing problems of regional water resources utilization; 2. Guiding ideology, basic principles and planning objectives; 3. Analysis of economic, social and ecological water demand; 4. Plan for water resources allocation; 5. Construction of water right system; 6. Main engineering measures; 7. Environmental impact arrangement.
Automatic monitoring data of groundwater level depth and salinity of three shallow groundwater observation Wells in ejin delta. Data contents include: observation well number, geographical coordinates, description of surface features, buried depth of groundwater level (unit: cm), salinity (unit: mS/cm). In terms of space, the dynamic monitoring of water and salt is set up in desert gobi area, natural oasis area and artificial oasis area in ejin delta, representing three typical underlying surface conditions.Since May 12, 2011, the frequency of observation has been 30 minutes.
YU Jingjie
Field survey data of ecological vegetation sample in ejin delta during the project implementation period. A sample of ecological vegetation survey near 31 groundwater salinity observation points in ejin delta.The main investigation items include: plant species, plant structure, number, height, base diameter, crown width, coverage, frequency, etc.Time: 2010 and 2011 (july-august).
YU Jingjie
Soil survey data corresponding to the ejin delta and the ecological vegetation sample during the project implementation period. Soil profile sampling corresponding to the ecological vegetation survey in ejin delta (5), 20 cm stratified sampling.Investigation items included: soil salinity, soil organic matter, C, N, P, etc., time: August 2011.
YU Jingjie
1. Data overview The sampling period of this data set was from July 19, 2012 to August 17, 2012. The location of the sampling point was near the original hongnigou outlet of the small cucurbitou watershed in the upper reaches of the heihe river, with the latitude and longitude of 99 ° 52 '25.3 "E, 38 ° 15' 37.97" 2. data content A soil profile with a depth of 2m was dug at the sampling point and a soil water collector was placed at depths of 60cm, 90cm and 140cm respectively.When soil water is collected by the soil collector, different depths of soil water can be obtained by extracting tubes of different depths with a 50ml disposable syringe. This data set contains the DOC value, ammonia nitrogen value, silica content and anion value of soil water at three different depths. Data acquisition method - ammonia nitrogen value was determined by using a hash DR2800 ultraviolet spectrophotometer;The DOC value was determined by the analytikjena multi N/C 3100 total nitrogen and carbon tester.The anion value was determined by Swiss wantong model 761/813 ion chromatograph.Cation is to use the model to the United States thermoelectric IRIS Intrepid Ⅱ XSPICP - AES determination.
SUN Ziyong, CHANG Qixin
1. Data overview The soil temperature monitoring point of the typical soil profile in the small basin of cucurbitou was set in the middle and lower part of the red mud ditch, and its geographical coordinates were 99 ° 52 '25.3 "E, 38 ° 15' 37.97" N. Soil Temperature was observed using HOBO Pendant® Temperature/Light Data Logger 64k-ua-002-64 Temperature recorder. 2. data content Soil temperature monitoring in typical soil profile of hongnigou is divided into seven layers, with depth distribution of 20cm, 40cm, 60cm, 80cm, 120cm, 160cm and 200cm.The frequency of observation is 1 time /15 minutes.The time range of observation data is from September 7, 2012 to May 6, 2013.
SUN Ziyong, CHANG Qixin
Data of field hydrogeological double-ring seepage test in 2012 in mamane mountain area, gansu province.The method adopted is the double ring method.Specific test process: fixed head water injection, observation record.According to the ring bottom ruler, keep the fixed head of water injection.Meanwhile, the injected water was observed according to the ruler on the injection plastic bucket, and the recorded time intervals were 5 minutes, 10 minutes, 20 minutes and 30 minutes respectively.Stable water seepage, that is, the completion of the experiment.The relevant permeability parameters are obtained according to darcy's law.
GUO Yonghai
In August 2011 to October, 2012 in gansu province during may to August mazong mountain region field hydrogeological investigation, for each of groundwater, surface water outcropping points, according to the requirements of sampling, collecting water samples of 500 ml, sealed bottle, tag sampling time, location, number, send relevant qualification of laboratory tests, groundwater, surface water chemical analysis testing data obtained.Cations: Na+,K+,Mg2+,Ca2+, PH;Anions: F-,Cl-,NO3-,SO42-,HCO3-,CO32-;Trace elements, etc.In order to understand the chemical distribution of surface water and groundwater in the ma mane shan research area.
GUO Yonghai
This data set contains the meteorological data of 45 regional stations in Zhangye area of Gansu Province from 2008 to 2009. There are two factors (air temperature and rainfall): Dongdashan forest farm and Anyang in Ganzhou district; Horseshoe temple in Sunan County; Longqu in Zhangye; Junma farm in Shandan; Mawei Lake in Gaotai; Banqiao in Linze. The observation of the three elements (wind direction, air temperature and rainfall) are: the Imperial City, the big river and recreation in Sunan County. The observation of the four elements (wind direction, wind speed, air temperature and rainfall) are: Tiancheng, Baba, luotuocheng, Xinba and Nanhua in Gaotai County; Pingchuan, Xinhua, nijiaying and yinggezui in Linze County; Jing'an, hongshawo forest farm, pingpingpingbao, Daman, alkali beach and shigangdun in Ganzhou district; Gushanzi, Longshoushan forest farm, Laojun, Liqiao, dongle, Junma first farm in Shandan County Liudun and junmachang in Qilian Mountain; Liuba, Sanbao, zhaizhaizhaizi, shuangshusi, haichaoba and dadonggan in Minle County; Xishui in Sunan County. The observation of the five factors (relative humidity, wind direction, wind speed, air temperature and rainfall) are: Yanzhishan forest farm in Shandan County; Minghua in Sunan County. The observation of the five factors (air pressure, wind direction, wind speed, air temperature and rainfall) are: Yanzhishan forest farm in Shandan County; Minghua in Sunan County. The six elements of observation (air pressure, humidity, wind direction, wind speed, air temperature and rainfall) are as follows: East top of dacha, dacha and crescent platform in Sunan County. The data recording unit shall comply with the ground meteorological observation specifications, and the data storage shall be expressed as an integer, as follows: ten times record of temperature expansion; ten times record of precipitation expansion; ten times record of wind speed expansion. The data format is ASCII text file.
Gansu meteorological bureau, Zhangye city meteorological bureau
This data set contains the observation data of Zhangye National Climate Observatory from 2008 to 2009. The station is located in Zhangye, Gansu Province, with longitude and latitude of 100 ° 17 ′ e, 39 ° 05 ′ N and altitude of 1456m. The observation items include: atmospheric wind temperature and humidity gradient observation (2cm, 4cm, 10cm, 20m and 30m), wind direction, air pressure, photosynthesis effective radiation, precipitation, radiation four components, surface temperature, multi-layer soil temperature (5cm, 10cm, 15cm, 20cm and 40cm), soil moisture (10cm, 20cm, 50cm, 100cm and 180cm) and soil heat flux (5cm, 10cm and 15cm). Please refer to the instruction document published with the data for specific header and other information.
Zhangye city meteorological bureau
In this dataset samples were obtained from groundwater outcrop points and surface water points through the field hydrogeological survey of mabongshan, and the analysis data of deuterium - oxygen - 18 and tritium were obtained by sending them to the laboratory with relevant qualification. This dataset can obtain the isotopic information of groundwater and surface water in the research area of the project, and provide data reference for the water circulation law in the research area.
GUO Yonghai
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