The dataset contains microbial amplicon sequencing data from a total of 269 ice samples collected from 15 glaciers on the Tibetan Plateau from November 2016 to August 2020, including 24K Glacier (24K), Dongkemadi Glacier (DKMD), Dunde Glacier (DD), Jiemayangzong Glacier (JMYZ), Kuoqionggangri Glacier (KQGR), Laigu Glacier (LG), Palung 4 Glacier (PL4), Qiangtang 1 Glacier (QT), Qiangyong Glacier (QY), Quma Glacier (QM), Tanggula Glacier (TGL), Xiagangjiang Glacier (XGJ), Yala Glacier (YA), Zepugou Glacier (ZPG), ZhufengDongrongbu Glacier (ZF). The sampling areas ranged in latitude and longitude from 28.020°N to 38.100°N and 86.28°E to 95.651°E. The 16s rRNA gene was amplified by polymerase chain reaction (PCR) using 515F/907R (or 515F/806R) primers and sequenced with the Illumina Hiseq2500 sequencing platform to obtain raw data. The selected primer sequences were "515F_GTGYCAGCMGCCGCGGTAA; 907R_CCGTCAATTCMTTTRAGTTT" "515F_GTGCCAGCMGCCGCGG; 806R_ GGACTACHVGGGTWTCTAAT". The uploaded data include: sample number, sample description, sampling time, latitude and longitude coordinates, sample type, sequencing target, sequencing fragment, sequencing primer, sequencing platform, data format and other basic information. The sequencing data are stored in sequence file data format forward *.1.fq.gz and reverse *.2.fq.gz compressed files.
LIU Yongqin
In order to describe the distribution pattern of genetic diversity of main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas (Pan third pole area), and clarify its related genetic background. In 2020, we extracted the total DNA from 266 global chicken blood, tissue and other DNA tissue samples, built a database and sequenced the whole genome. At the same time, we downloaded the published chicken genome data, and carried out population analysis of 863 chicken genomes, so as to provide basic data for exploring the historical events of domestication, migration and expansion of domestic chickens in the pan third pole region, and further explore the adaptation mechanism of domesticated animals to harsh environments such as drying. Articles related to this data set have been published. All data in this data set can be downloaded online from fastq, BAM, VCF and SNP files.
PENG Minsheng
In order to integrate the pan third pole domestic chicken data, build the "global domestic chicken genome database (chicken2k)", provide basic data for the international research on the origin, domestication and selection of domestic chickens, and provide scientific guidance for the breeding and improvement of new domestic chicken varieties. In 2022, this sub project cooperated with the Animal Branch of the Germplasm Bank of Wild Species, Chinese Academy of Sciences, to apply for the use of genetic samples of domestic animals in Southeast Asia collected and preserved in recent years, and select representative individuals to carry out genetic diversity assessment. This data set contains 224 blood and tissue samples of domestic chickens and Hongyuan chickens from Southeast Asian countries (Laos, Thailand, Myanmar and Vietnam) collected by the Animal Germplasm Resource Bank. This data set contains basic sample information such as sample species, variety, detailed sampling place, sample type, collection time, collector and storage method, which are stored in Excel form.
PENG Minsheng
This sub project aims to conduct comprehensive investigation and Research on amphibian groups in weak and key areas of the Qinghai Tibet Plateau, namely the Himalayas and Hengduan Mountains, obtain background data of regional animal diversity, collect a batch of specimens, collect specimens of species and environmental photos, and analyze and study amphibian diversity and its spatial pattern. In 2021, 500 local amphibian tissue samples were collected in Motuo County, Nyingchi Prefecture, Tibet Autonomous Region. This data set includes 1 tissue sample table, 1 specimen sample table and 900 photos. The sample information table includes basic sample information such as species, variety, detailed collection, sample type, collection time, collector and storage method, which are stored in the form of Excel. Photos, stored in JPG format.
YUAN Zhiyong
In order to investigate the distribution of birds in Tibet, the sub project sub project 2019qzkk05010108 conducted an important investigation on the background resources of birds in Beibeng Township, Motuo County in 2021, especially conducted an in-depth and detailed bird investigation on the understory birds in xirang village, Didong village and green village near the illegal Indian controlled area, and analyzed the composition of the understory bird community at medium and low altitude. A total of 717 bird specimens were collected, including 73 bird species. The results showed that the main dominant species in the investigated altitude range (600 ~ 1500m, mainly in the middle and low altitude) were stachyris nigriceps, schoeniparus cinereus, cyanoderma chrysaeum and alcippe nipalensis). There are also occasional species such as Zoothera monticola. This data set includes specimen photos, habitat photos, working photos, specimen information table and tissue sample information table.
WU Fei
In order to master the species composition, floristic characteristics and host information of plateau agricultural and animal husbandry elephants and related natural enemy insects such as Coleoptera, Neuroptera and Diptera, establish a DNA bar code rapid identification system of plateau agricultural and animal husbandry natural enemy insects, evaluate the current situation of natural enemy resources, and put forward suggestions for the sustainable utilization of natural enemy insects. The sub project 2019qzkk05010606 carried out the investigation of natural enemy insect resources in key agricultural and pastoral areas, bulk crop related elephants, Coleoptera, Neuroptera and Diptera on the Qinghai Tibet Plateau, the construction of natural enemy insect species diversity database, and the evaluation of the current situation and sustainable utilization of natural enemy resources. During 2020, the Tibet Autonomous Region, the farming pastoral ecotone, the Farming Forestry ecotone, and the hinterland of farming and pastoral areas in Yunnan Province will carry out the investigation of key groups of natural enemy insects such as Coleoptera, Neuroptera and Diptera, collect samples, biological information and ecological environment information, systematically sort out the samples of natural enemy insects according to the standards and norms, and effectively preserve them, Carry out species morphological identification and obtain DNA bar code information, integrate species geographical distribution, host information, ecological pictures and other information, and build a natural enemy species diversity information database; Evaluate the current situation of natural enemy resources and put forward suggestions for sustainable utilization.
LIU Ning
In order to master the species composition, floristic characteristics and host information of plateau agricultural and animal husbandry elephants and related natural enemy insects such as Coleoptera, Neuroptera and Diptera, establish a DNA bar code rapid identification system of plateau agricultural and animal husbandry natural enemy insects, evaluate the current situation of natural enemy resources, and put forward suggestions for the sustainable utilization of natural enemy insects. The sub project 2019qzkk05010601 carried out the investigation of natural enemy insect resources in key agricultural and pastoral areas, bulk crop related elephants, Coleoptera, Neuroptera and Diptera on the Qinghai Tibet Plateau, the construction of natural enemy insect species diversity database, and the evaluation of the current situation and sustainable utilization of natural enemy resources. During 2020, the Tibet Autonomous Region, the farming pastoral ecotone, the Farming Forestry ecotone, and the hinterland of farming and pastoral areas in Yunnan Province will carry out the investigation of key groups of natural enemy insects such as Coleoptera, Neuroptera and Diptera, collect samples, biological information and ecological environment information, systematically sort out the samples of natural enemy insects according to the standards and norms, and effectively preserve them, Carry out species morphological identification and obtain DNA bar code information, integrate species geographical distribution, host information, ecological pictures and other information, and build a natural enemy species diversity information database; Evaluate the current situation of natural enemy resources and put forward suggestions for sustainable utilization.
QIAO Gexia
In order to analyze the animal diversity pattern of the Qinghai Tibet Plateau and establish the corresponding animal specimen database. The sub project 2019qzkk05010113 was concentrated in the West Tianshan Mountain of Xinjiang in 2021. A total of 200 specimens and tissue samples of local wild animals, such as common vole and Apodemus agrarius, were collected. The solid samples include 200 specimen information tables and 500 photos, such as animal individuals, skins and tissues. This data set includes 1 data set specification table, 1 specimen information table and 1 tissue sample information table. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel. Photos, stored in JPG format.
JIANG Xuelong
In order to analyze the animal diversity pattern of the Qinghai Tibet Plateau and establish the corresponding animal specimen database. The sub project 2019qzkk05010110 was concentrated in Ya'an City, Sichuan Province and Ganzi Tibetan Autonomous Prefecture in 2021. A total of 200 specimens and tissue samples of local wild animals, such as Apodemus agrarius and social mice, were collected. The solid samples include animal individuals, skins, tissues, etc. This data set contains 200 sample information tables and 374 photos. This data set includes 1 data set specification table, 1 specimen information table and 1 tissue sample information table. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel. Photos, stored in JPG format.
CHEN Zhongzheng
1) Data content: changes in genetic diversity of 10 amphibians and reptiles on the Qinghai Tibet Plateau in the face of future climate change. 2) Data source and processing method: Based on the bar code data of 10 amphibians and reptiles on the Qinghai Tibet Plateau, combined with SDM, MPTP approach and other software, the genetic diversity and distribution in 2050, 2070 and 2090 in the future are constructed. 3) Data quality description: the data quality is verified, and the data analysis personnel are strictly trained in the laboratory. 4) Results and prospects of data application: it is found that amphibians and reptiles distributed in the north of Qinghai Tibet Plateau need more attention in protection.
SHEN Wenjing
In order to describe the distribution pattern of genetic diversity of main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, clarify their related genetic background, and establish the corresponding genetic resource bank. In 2021, we collected a total of 267 samples of blood, heart, liver, spleen, lung, kidney, muscle, fat, large intestine, small intestine, stomach and testis of large forehead cattle (Dulong cattle) in Yunnan Province. This data set contains basic sample information such as sample species, species, detailed sampling place, gender, sample type, collection time, collector and storage method, as well as individual photos. The solid samples are stored in the wildlife germplasm resource bank and Animal Germplasm Resource Bank in Southwest China.
LI Yan
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