The dataset of survey at the poplar sampling plot was obtained in the Linze station foci experimental area. Observation items included: (1) soil profile moisture and temperature (0-5cm, 0-5cm, 10-20cm, 20-40cm and 40-60cm) with photos measured twice by the cutting ring method (50cm^3, each layer), once by ML2X Soil Moisture Tachometer and the probe thermometer (15cm, twice each layer) on Jun. 3, 2008. Data were archived as Excel files. (2) shallow layer soil moisture (0-5cm) measured once by the cutting ring method (50cm^3, once each point) and twice by ML2X Soil Moisture Tachometer on Jun. 4, 2008. 13 points were selected and data were archived as Excel files. (3) LAI by TRAC on Jul. 20, 2008. Data were archived as Excel files. (4) roughness measured by the roughness plate together with the digital camera. 18 points were selected and data were archived in JPG format format. (5) forest investigation of Populus gansuensis from Jun. 5-13, 2008: coordinates, the diameter at breast height and the crown size by the measuring tape, full height by TruPulse200. 408 trees were selected and data were archived as Excel files. See the metadata record “WATER: Dataset of setting of the sampling plots and stripes in the Linze station foci experimental area” for more information of the quadrate locations.
BAI Yanfen, DING Songchuang, HAO Xiaohua, PAN Xiaoduo, Qian Jinbo, SONG Yi, WANG Yang, WANG Zhixia, ZHU Shijie
The dataset of vegetation cover fraction observations was obtained by the self-made instrument and the camera at a height of 2.5m-3.5m above the ground in the Yingke oasis, Huazhaizi desert steppe and Biandukou foci experimental areas on May 20, 24, 25, 28 and 30, Jun. 11, 14, 15, 21, 23, 24, 27 and 30, and Jul. 2, 2008. Observations were carried out in Yingke oasis maize field, Yingke oasis wheat field, Huazhaizi desert No. 1 and 2 plots, the rape field, the barley field and grassland in Biandukou. A pole with known length was put in each photo to determine the size of the photo. GPS data was used for the location and the technology LAB was used to retieve the coverage of the green vegetation. Besides, surrounding environment was also recorded. The dataset included the primary collected vegetation images and retrieved fraction of vegetation coverage.
QIAN Yonggang, REN Huazhong, WANG Haoxing, WANG Jindi, WANG Tianxing, YAN Guangkuo, ZHANG Wuming
The dataset of the discrimination of C3/C4 species was obtained by the handheld GPS and the digital camera in the Linze station foci experimental area on Jul. 10, 2008. Data fields included Gps, Longitude, Latitude, Photo_num and Describe (descriptions on C3/C4 vegetation and photos).
CHENG Zhanhui, Liu Liangyun
The dataset of water content of forest canopy components (the twig and the leaf) measurements was obtained at the super site (100m×100m) around the Dayekou Guantan forest station on Jun. 5, 2008. The sample tree was selected according to different diameters at breast height. 5 diameter classes were divided and in each class, 10 trees were selected and altogether 30 trees were selected as sampling trees. Branches in different parts were picked by the tree pruner and the twig and the leaf were separated manually, whose green weight was measured by the scales on the scene and dry weight by oven drying in the lab. Those provide reliable data for the reconstruction of the 3D structure of the forest scene, and for modelling active and passive remote sensing mechanisms and the simulation of remote sensing images.
BAI Lina, TIAN Xin, WANG Bengyu, CHEN Erxue
In the middle of July, 2011, 1. Elaeagnus angustifolia, 2. Blister. Using Li-6400 portable photosynthesis system (li-cor, USA) and li-3100 leaf area meter, the photosynthetic physiological characteristics of desert plants were observed. The symbols in the observation data have the following meanings: Obs, number of observations;Photo, net photosynthetic rate, moles of CO2 times m minus 2 times s minus 1; Cond, stomatal conductance, mol H2O•m -- 2•s -- 1;Ci, intercellular CO2 concentration, moles of CO2 times mol-1; Trmmol, transpiration rate, mmol H2O•m -- 2•s -- 1;Vpdl, water vapor pressure deficit, kPa; Area, leaf Area, cm2;Tair, atmospheric temperature, ℃; Tleaf, leaf surface temperature, ℃;CO2R, CO2 concentration in the reference chamber, moles of CO2•mol-1; CO2S, sample chamber CO2 concentration, moles of CO2•mol-1;H2OR, water in the reference chamber, mmol H2O•mol-1; H2OS, sample chamber moisture, mmol H2O•mol-1;PARo, photon flux density, mole •m -- 2•s -- 1; Rh-r, reference room air relative humidity, %;Rh-s, relative humidity of air in sample room, %; PARi, photosynthetic effective radiation, moles •m -- 2•s -- 1;Press, atmospheric pressure, kPa; Others are the state parameters of the instrument at the time of measurement.
SU Peixi
The data is the digitization of the Heihe River basin part of the 1:1 million Vegetation Atlas of China, 1:1000, 000 Vegetation Atlas of China is edited by academician Hou Xueyu, a famous vegetation ecologist (Hou Xueyu, 2001). It is jointly compiled by more than 250 experts from 53 units such as research institutes of Chinese Academy of Sciences, relevant ministries and commissions, relevant departments of various provinces and regions, colleges and universities. It is another summative achievement of vegetation ecologists in China over 40 years after the publication of monographs such as vegetation of China Basic map of natural resources and natural conditions of the family. It is based on the rich first-hand information accumulated by vegetation surveys carried out throughout the country over the past half century, and the materials obtained by modern technologies such as aerial remote sensing and satellite images, as well as the latest research achievements in geology, soil science and climatology. It reflects in detail the distribution of vegetation units of 11 vegetation type groups, 796 formations and sub formations of 54 vegetation types, horizontal and vertical zonal distribution laws, and also reflects the actual distribution of more than 2000 dominant species of plants, major crops and cash crops in China, as well as the close relationship between dominant species and soil and ground geology. The atlas is a kind of realistic vegetation map, reflecting the recent quality of vegetation in China.
HOU Xueyu
The data is a fisheye photo above the interception barrel of the Picea crassifolia plot in the Tianlaochi small watershed of Qilian Mountain. The plot has a latitude and longitude of 38.44N, 99.91E, and an altitude of 2793m. Photo DSC_0008——DSC_0097 corresponds to Fisheye photos above interception barrels 1 to 90 respectively. The camera is directly above the interception barrel and the lens is 1m above the ground. It is used to estimate the cover or LAI of Qinghai spruce forest, and the pictures are processed with Gap Light Analyzer software.
ZHAO Chuanyan, MA Wenying
At the end of September and the beginning of October, 2011, a year-end ecological survey was carried out in heihe river basin for plants of different desert types to stop growing. There are altogether 8 survey and observation fields, which are: piedmont desert, piedmont gobi, middle reaches desert, middle reaches gobi, middle reaches desert, lower reaches desert, lower reaches gobi and lower reaches desert, with a size of 40m×40m. Three 20m×20m large quadrats were fixed in each observation field, named S1, S2 and S3, and regular shrub surveys were conducted.Each large quadrat was fixed with 4 5m x 5m small quadrats, named A, B, C, D, for the herbal survey.
SU Peixi
In mid-july 2011, photosynthetic organs (leaves or assimilating branches) of typical desert plants were collected and brought back to the laboratory in a liquid nitrogen tank for determination. The analysis indexes mainly include soluble protein unit: mg/g;Free amino acid unit: g/g;Chlorophyll content unit: mg/g;Superoxide dismutase (SOD) unit: U/g FW;Catalase (CAT) unit: U/(g•min);POD unit: U/(g•min);Proline (Pro) unit: g/g; Soluble sugar unit: g/g;Malondialdehyde (MDA) is given in moles per liter.
SU Peixi
A small lysimeter was made by ourselves, which simulated the natural conditions and selected typical desert plants as the object to study the water consumption and its law. Repeat 3 times for each plant.
SU Peixi
In mid July 2011, the photosynthetic organs (leaf or assimilating branches) of typical desert plants were collected and determined by laboratory. The indicators include: leaf water potential, total leaf water content, relative water content, dry weight water content, leaf dry matter content, specific leaf area, specific leaf volume, free water, bound water, etc.
SU Peixi
This data includes three parts of data, namely shrub water holding experiment, shrub interception experiment and shrub transpiration experiment data. Shrub water holding experiment: select the two shrub types of Caragana jubata and Potentilla fruticosa, respectively pick the branches and leaves of the two vegetation types, weigh their fresh weight, carry out water holding experiment, measure the saturated weight of branches and leaves, dry weight of branches and leaves, dry weight of branches and leaves after completion, and finally obtain the data of branches, leaves and total water holding capacity. Shrub interception experiment: two shrubs, Caragana jubata and Potentilla fruticosa, were also selected and investigated. 30 rain-bearing cups were respectively arranged under the two shrubs. after each rainfall, penetration rainfall was measured and observed from June 1, 2012 to September 10, 2012. Shrub Transpiration Experiment: Potentilla fruticosa on July 14, Caragana jubata on August 5, Salix gilashanica on August 15, 2012. The measurement is made every hour according to the daily weather conditions.
ZHAO Chuanyan, MA Wenying
China 1:100000 data of land use is a major application in the Chinese Academy of Sciences "five-year" project "the national resources and environment remote sensing macroscopic investigation and study of dynamic organized 19 Chinese Academy of Sciences institute of remote sensing science and technology team, by means of satellite remote sensing, in three years based on Landsat MSS, TM and ETM remote sensing data established China 1:100000 images and vector of land use database.The main contents include: China 1:100,000 land use data;China 1:100,000 land use graph data and attribute data. The data was directly clipped from China's 1:100,000 land-use data.A hierarchical land cover classification system was adopted for the land use data of heihe basin of 1:100,000, and the whole basin was divided into 6 primary categories (arable land, forest land, grassland, water area, urban and rural areas, industrial and mining areas, residential land and unused land) and 26 secondary categories.The data type is vector polygon, which is stored in Shape format.There are two types of data projection: WGS84/ALBERS;Data coverage covers the new heihe watershed boundary (lack of outer Mongolia data). Land use classification attributes: The first class type and the second class type attributes encode the spatial distribution position Cultivated paddy field 113 is mainly distributed in alluvial plain, basin and valley Cultivated paddy field 112 distributed in hilly valley narrow valley platform or beach (with irrigation conditions) Cultivated paddy field 111 is mainly distributed in mountain valley narrow valley platform or beach (with better irrigation conditions) Arable land 124 is mainly distributed in mountainous areas, the slope is generally more than 25 degrees (belongs to the steep slope hanging land), should be returned to forest. Cultivated dry land 123 is mainly distributed in basins, piedmont belts, river alluvial, diluvial or lacustrine plains (water shortage and poor irrigation conditions). Cultivated dry land 122 is mainly distributed in hilly areas (shaanxi, gan, ning, qing).In general, the plot is distributed on gentle slopes and x and sockets of hills. Arable land 121 is mainly distributed in the mountainous area, with an elevation of 4000 meters below the slope (gentle slope, mountainside, steep slope platform, etc.) and mountain front belt. Woodlands have woodlands (trees) 21 mainly distributed in the mountains (below 4000 meters above sea level) or in the slope, valley two slopes, mountain tops, plains.In qinghai nanshan, qilian mountains are. Woodland shrub 22 is mainly distributed in the higher mountain areas (below 4500 m), most of the distribution of hillside and valley and sand. Forest dredging 23 mainly distributed in the mountains, hills, plains and sandy land, gobi (soil, gravel) edge. Other woodlands 24 are mainly distributed in the oasis ridge, river, roadside and rural residential areas around. Grassland 31 is generally distributed in mountainous areas (gentle slopes), hills (steep slopes) and interriver beaches, gobi desert, sandy hills, etc. The covered grassland 32 is mainly distributed in dry places (next door low-lying land and sandy hills, etc.). Grassland low cover grassland 33 mainly grows in drier places (loess hills and sandy edges). The river channel 41 is mainly distributed in the plain, the cultivated land between the rivers and the valleys in the mountains. Water lakes are mainly distributed in low-lying areas. The reservoirs are mainly distributed in the intermountain lowlands and intersandy hills in qinghai province. Water area glaciers and permanent snow 44 mainly distributed in the plain, the valley between the river, there are surrounding residents and arable land. Waters and beaches are mainly distributed on the top of (over 4000) mountains.
WANG Jianhua, LIU Jiyuan
The data are from 2011 to 2012. A 30m×30m Picea crassifolia canopy interception sample plot was set up in the Picea crassifolia sample plot at an altitude of 2800m m. A siphon raingauge model DSJ2 (Tianjin Meteorological Instrument Factory) was set up on the open land of the river about 50m from the sample plot to observe the rainfall outside the forest and its characteristics. Penetrating rain in the forest adopts a combination of manual observation and automatic observation. Automatic observation is mainly realized through a penetrating rain collection system arranged in the interception sample plot, which consists of a water collecting tank and an automatic recorder. Two 400cm×20cm water collecting tanks are connected with DSJ2 siphon rain gauge, and the change characteristics of penetrating rain under the forest are continuously recorded by an automatic recorder. Due to the spatial variability of the canopy structure of Picea crassifolia forest in the sample plot, a standard rainfall tube for manual observation is also arranged in the sample plot to observe the penetrating rain in the forest. Ninety rainfall tubes with a diameter of 20cm are arranged in the sample plot at intervals of 3m. After each precipitation event ends and the penetrating rain in the forest stops, the amount of water in the rain barrel will be emptied and the penetrating rain in the barrel will be measured with the rain cup.
ZHAO Chuanyan, MA Wenying
Investigation of plant sample plots can reflect the structure and distribution of plant communities, the declining succession of plant communities and their interrelation with environmental changes, reveal the ecological damage process in the lower reaches of the Tarim River, and provide scientific basis for the environmental remediation of the Tarim River Basin in the large-scale development of the western part of the country. According to the difference of species composition of plant communities in different sections of 9 monitoring sections in the lower reaches of Tarim River, plant sample plots are set up along the direction perpendicular to the river course in each monitoring section. Due to the different vegetation growth in each section, the size and number of sample plots are not equal. Among them, the sample plot of 5m×5m is arranged on the section of the herbaceous community. 30m×30m sample plots are arranged on the section where vegetation grows sparsely or is basically free of herbaceous plants, and 4 15m× 15 m arbor and shrub sample plots are arranged at intervals of 15 m; 50m×50m sample plots are arranged on the section where arbor, shrub and grass vegetation all occupy a certain proportion. In each plot of 50×50m, four plots of 25m×25m are set at 25m intervals to record the individual number, coverage, DBH, basal diameter, height and crown width of each tree (or shrub). At the same time, 4 small sample plots of 5m×5m are set up in each sample plot to record the individual number, coverage, height and other indicators of each herbaceous plant, and GPS is used to locate and record the altitude and longitude and latitude of each sample plot. Data content includes: 1. word Document for Statistics of Plant Sample Land Survey Data from 2000, 2002 to 2007 2. 2000 Inventory of Plant Sample Sites in Lower Reaches of Tarim River (Akdun, Yahopumahan, Yingsu, Abodah, Keldayi Section Vegetation Coverage, Canopy Density, Root Weight, etc.) excel Table 3. excel Table of Plant Sample Plot Survey in Lower Reaches of Tarim River in August 2002 (Data on Individual Number, Crown Width, Plant Height, Density and Coverage of Plants in Akdun, Yingsu, Khaldayi, Arakan and Shidaoban Sections) 4. 2003 Inventory of Plant Sample Sites in Lower Reaches of Tarim River (Data on Individual Number, Crown Width, Plant Height, Density and Base Diameter of Plants in Lower Reaches of Tahe River and Herbaceous Biomass in Akerdun Section) excel Table 5. In September 2004, the lower reaches of the Tarim River plant sample plot questionnaire (data of individual number, crown width, plant height, basal diameter (or DBH), coverage and biomass) excel table of the lower reaches of the Tarim River in Yahefu Mahan, Yingsu, Abodah Le, Khaldayi, Tugamale, Arakan, Yiganbuma and Kaogan sections 6. In July 2005, the lower reaches of Tarim River plant sample plot questionnaire (9 monitoring sections in the lower reaches of Tahe River and data of individual number, crown width, plant height, basal diameter (or DBH) and coverage of plants in taitema lake, and herbaceous biomass data in Akerdun section) excel table 7. In July 2006, the lower reaches of Tarim River plant sample plot questionnaire (the number of individual plants, crown width, plant height, basal diameter (or DBH) and herbaceous biomass data of Akerdun section in 9 monitoring sections in the lower reaches of Tahe River) excel table 8. July 2007, the lower reaches of Tarim river plant sample plot questionnaire (the number of individual plants, crown width, plant height, basal diameter (or DBH) and herbaceous biomass data of akdun section in 9 monitoring sections in the lower reaches of Tahe river) excel table
CHEN Yaning, HAO Xingming, WU Lizong
On August 6, 2004, the institute of cold and drought, Chinese academy of sciences, organized a remote sensing experiment in the upper reaches of the heihe river basin, which obtained soil survey data of 14 sections, DEM of 1:500 scale in the drainage ditch basin, spectral data of typical features and synchronous ground observation data of dapingding TM and QuickBird satellite.It mainly includes: 1) spectral measurement data of typical ground objects The data mainly includes in continental river basin in linze county comprehensive research station near the station (hereinafter referred to as linze) of elaeagnus angustifolia, two poplars, tamarisk, bark, ephedra, sand, alfalfa, corn, cotton and salinization land spectra and dew ditch valley concept-people mei, grass, moss, alpine meadow grass, sword leaf thorns son, the spectra of soil and rock. 2) soil profile survey data Valley in line according to the altitude and vegetation types were set up 12 soil profile, and also in front of the row of dew ditch forest weather stations and linze weather station set up a soil profile 1, 14 were measured profile of soil moisture content, bulk density, adhering sand content and soil spectrum, dew ditch forest top weather stations and linze profile is measuring the thermal conductivity of soil and water parameters. 3) field measurement data of biophysical parameters of typical ground objects Standing near the corn, cotton, including linze small pine, alfalfa, and leaf area index measurement data of ephedra row dew in different heights with leaf photosynthesis, leaf area index data and vegetation features data (photosynthetic rate, stomatal conductance, intercellular CO2 concentration, leaf transpiration rate, leaf temperature) and the corresponding environmental factor data (air temperature, air relative humidity and atmospheric CO2 concentration, air, water content, atmospheric pressure, solar total radiation, photosynthetic active radiation). 4) ground synchronization test of remote sensing by large flat-topped satellite The simultaneous observation experiment of TM and QuickBird satellite was carried out in a relatively flat grass area (big flat roof) beside the drainage ditch watershed.On July 27, 2004, spectra, above-ground biomass and leaf area were measured at intervals of 15 meters in a 150m×150m quadrangular at a large flat roof.
LI Xin, RAN Youhua, HUANG Chunlin, QI Yuan, LU Ling, LI Jing, JING Zhefang, PENG Hongchun, Li Haiying, WANG Shugong
This data was compiled by Qiu Baoming, Gao Qianzhao, Peng Qilong, etc. of Lanzhou Desert Research Institute, Chinese Academy of Sciences, and published by Xi'an map publishing house in 1988 (Qiu Baoming, etc., 1988). The grassland is mainly divided into eleven categories: swamp grassland, low humidity grassland, plain desert grassland, plain semi desert grassland, desert riverside sparse forest shrub grassland, mountain desert grassland, mountain semi desert grassland, mountain grassland grassland, mountain meadow grassland, mountain meadow grassland, mountain shrub meadow grassland and ancillary grassland. Property fields include: Grassland code, type, and subclass.
Chou Baoming, Peng Qilong, Gao Qianzhao
Vegetation functional type (PFT) is the combination of large plant species according to the ecosystem function of plant species and the way of resource utilization. Each plant functional type shares similar plant properties, which simplifies the diversity of plant species to the diversity of plant function and structure.Vegetation functional types have been used in the dynamic global vegetation model (DGVM) to predict changes in ecosystem structure and function under global change scenarios.The 1km vegetation functional pattern map of heihe basin is based on the 1km land cover map of heihe basin (MICLCover subset of heihe basin), and is divided by using the vegetation functional climate rules proposed by Bonan et al. (2002).The climate data utilize the 0.1 degree atmospheric drive data of he jie and Yang kun, developing China region from 1981 to 2008.This map can be used in the land surface process model of heihe river basin.
RAN Youhua
In the ecosystem, soil and vegetation are two interdependent factors. Plants affect soil and soil restricts vegetation. On the one hand, there are a lot of nutrients such as carbon, nitrogen and phosphorus in the soil. On the other hand, the availability of soil nutrients plays a key role in the growth and development of plants, directly affecting the composition and physiological activity of plant communities, and determining the structure, function and productivity level of ecosystems. Soil moisture content (or soil moisture content): In the 9 sections from Daxihaizi to taitema lake in the lower reaches of Tarim River, plant sample plots are set in the direction perpendicular to the river channel according to the arrangement of groundwater level monitoring wells. Dig one soil profile in each sample plot, collect one soil sample from 0-5 cm, 5-15 cm, 15-30 cm, 30-50 cm, 50-80 cm, 80-120 cm and 120-170cm soil layers from bottom to top in each profile layer, each soil sample is formed by multi-point sampling and mixing of corresponding soil layers, each soil layer uses aluminum boxes to collect soil samples, weighs wet weight on site, and measures soil moisture content (or soil moisture content) by drying method. Soil nutrient: the mixed soil sample is used for determining soil nutrient after removing plant root system, gravel and other impurities, air-drying indoors and sieving. Organic matter is heated by potassium dichromate, total nitrogen is treated by semi-micro-Kjeldahl method, total phosphorus is treated by sulfuric acid-perchloric acid-molybdenum antimony anti-colorimetric method, total potassium is treated by hydrofluoric acid-perchloric acid-flame photometer method, effective nitrogen is treated by alkaline hydrolysis diffusion method, effective phosphorus is treated by sodium bicarbonate leaching-molybdenum antimony anti-colorimetric method, effective potassium is treated by ammonium acetate leaching-flame photometer method, PH and conductivity are measured by acidimeter and conductivity meter respectively (water to soil ratio is 5: 1). Soil water-soluble total salt was determined by in-situ salinity meter. Drought stress is the most common form of plant adversity and is also the main factor affecting plant growth and development. Plant organs will undergo membrane lipid peroxidation under adverse circumstances, thus accumulating malondialdehyde (MDA), the final decomposition product of membrane lipid peroxide. MDA content is an important indicator reflecting the strength of membrane lipid peroxidation and the damage degree of plasma membrane, and is also an important parameter reflecting the damage of water stress to plants. At the same time, under adverse conditions, the increased metabolism of reactive oxygen species in plants will lead to the accumulation of reactive oxygen species or other peroxide radicals, thus damaging cell membranes. Superoxide dismutase (SOD) and peroxidase (POD) in plants can remove excess active oxygen in plants under drought and other adversities, maintain the metabolic balance of active oxygen, protect the structure of the membrane, and finally enhance the resistance of plants to adversities. The analysis samples take Populus euphratica, Tamarix chinensis and Phragmites communis as research objects. According to the location of groundwater monitoring wells, six sample plots are set up starting from the riverside, with an interval of 50 m between each sample plot, which are sample plots 1, 2, 3, 4, 5 and 6 in turn. Fresh leaves of plants are collected, stored at low temperature, and pretreated (dried or frozen) on the same day. PROline (Pro), cell membrane system protective enzymes superoxide dismutase (SOD) and peroxidase (POD) were tested indoors. Preparation of enzyme solution: weigh 0.5g of fresh material and add 4.5mL pH7.8 with ph 7.8. The materials were homogenized in a pre-frozen mortar, which was placed in an ice bath. Centrifuge at 10000 r/min for 15 min. The supernatant was used for determination of superoxide dismutase, peroxidase and malondialdehyde (MDA). PRO determination: put 0.03 g of material into a 20 mL large test tube, add 10mL ammonia-free distilled water, seal it, put it in a boiling water bath for 30min, cool it, filter, filtrate 5 mL+ ninhydrin 5 mL, develop color in boiling water for 60min, and extract with toluene. The extract was colorized with Shimadzu UV-265 UV spectrophotometer at 515 nm. SOD activity was measured by NBT photoreduction. The order of sample addition for enzyme reaction system is: pH 7.8 PBS 2.4mL+ riboflavin 0.2 mL+ methionine 0.2 mL+EDTA0.1 mL+ enzyme solution 0.1 mL+NBT0.2 mL. Then the test tube was reacted under 40001ux light for 20 min, and photochemical reduction was carried out. SOD activity was measured at 650 nm wavelength by UV-265 ultraviolet spectrophotometer. POD activity determination: the reaction mixture was 50 ml PBS with pH 6.0+28 μ L guaiacol+19 UL30% H2O2. 2 mL of reaction mixture +1 mL of enzyme solution, immediately start timing, reading every 1 min, reading at 470 nm. Determination of chlorophyll: ethanol acetone mixed solution method. After cutting the leaves, the mixed solution of 0.2 g and acetone: absolute ethanol = 1: 1 was weighed as the extraction solution. After extracting in the dark for 24 h, the leaves turned white and chlorophyll was dissolved in the extraction solution. The OD value of chlorophyll was measured by spectrophotometer at 652nm. Determination method of soluble sugar: phenol sulfate method is adopted. (1) The standard curve is made by taking 11 20 ml graduated test tubes, numbering them from 0 to 10 points, and adding solution and water according to Table 1 respectively. Then add 1 ml of 9% phenol solution to the test tube in sequence, shake it evenly, then add 5 ml of concentrated sulfuric acid from the front of the tube for 5 ~ 20 s, the total volume of the colorimetric solution is 8 ml, and leave it at constant temperature for 30 minutes for color development. Then, with blank as control, colorimetric determination was carried out at 485 nm wavelength. With sugar as abscissa and optical density as ordinate, a standard curve was drawn and the equation of the standard curve was obtained. (2) Extraction of soluble sugar: fresh plant leaves are taken, surface dirt is wiped clean, cut and mixed evenly, 0.1-0.3 g are weighed, 3 portions are respectively put into 3 calibration test tubes, 5-10 ml distilled water is added, plastic film is sealed, extraction is carried out in boiling water for 3O minutes, the extraction solution is filtered into a 25 ml volumetric flask, repeated flushing is carried out, and the volume is fixed to the calibration. (3) Absorb 0.5 g of sample solution into the test tube, add 1.5 ml of distilled water, and work out the content of soluble sugar in the same way as the standard curve. The amount of solution and water in each test tube Pipe number 0 1-2 3-4 5-6 7-8 9-10 1.100μg/L sugar solution 0.20 0.40 0.60 1.0 2. water/ml 2.0 1.8 1.6 1.4 1.2 1.0 3. Soluble sugar content/μ g 0 20 40 60 80 100 Determination of malondialdehyde: thiobarbituric acid method. Fresh leaves were cut to pieces, 0.5 g was weighed, 5% TCA5 ml was added, and the homogenate obtained after grinding was centrifuged at 3 000 r/rain for 10 rain. Take 2 ml supernatant, add 0.67% TBA 2 ml, mix, boil in 100 water bath for 30 rain, cool and centrifuge again. Using 0.67% TBA solution as blank, the OD values at 450, 532 and 600 nm were determined. Methods for analysis and testing of plant hormones (GA3, ABA, CK, IAA): 0.1 0.005 g plant samples were taken and ground in liquid nitrogen. 500μl methanol was extracted overnight at 4℃. Centrifuge the sample and freeze-dry the supernatant. 30μl10%% CH3CN dissolved the sample. 10μl of sample solution was analyzed by HPLC. The external standard method was used to quantify plant hormones. Standard plant hormones were purchased from sigma Company. See (Ruan Xiao, Wang Qiang, et al., 2000, Journal of Plant Physiology.26 (5), 402-406) for analysis methods.
CHEN Yaning, HAO Xingming
The dataset of ground truth measurements synchronizing with airborne Polarimetric L-band Multibeam Radiometer (PLMR) mission was obtained in upper reaches of the Heihe River Basin on 1 August, 2012. PLMR is a dual-polarization (H/V) airborne microwave radiometer with a frequency of 1.413 GHz, which can provide multi-angular observations with 6 beams at ±7º, ±21.5º and ±38.5º. The PLMR spatial resolution (beam spot size) is approximately 0.3 times the altitude, and the swath width is about twice the altitude. The measurements were conducted along two transects respectively located at the west and east branches of the Babaohe River and two sampling plots in the A’rou foci experimental area. Along the transects, soil moisture was sampled at every 50 m in the west-east direction. In order to keep the ground measurements following the airborne mission as synchronous as possible in temporal, measurements were made discontinuously. In the A’rou foci experimental area, two sampling plots were identified with areas of 1.5 km × 0.6 km and 0.85 km × 0.6 km. In each plot, soil moisture was sampled at every 50 m in the west-east direction and 100 m in the north-south direction. Steven Hydro probes were used to collect soil moisture and other measurements. Concurrently with soil moisture sampling, vegetation properties were measured at some typical sampling plots. Observation items included: Soil parameters: volumetric soil moisture (inherently converted from measured soil dielectric constant), soil temperature, soil dielectric constant, soil electric conductivity. Vegetation parameters: biomass, vegetation water content, canopy height. Data and data format: This dataset includes two parts of measurements, i.e. soil and vegetation parameters. The former is as shapefile, with measured items stored in its attribute table. The measured vegetation parameters are recorded in an Excel file.
LI Xin, MA Mingguo, WANG Shuguo
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