This data includes bacterial 16S ribosomal RNA gene sequence data from 25 lakes in the middle of the Qinghai Tibet Plateau. The sample was collected from July to August 2015, and the surface water was sampled three times with a 2.5 liter sampler. The samples were immediately taken back to the Ecological Laboratory of the Beijing Qinghai Tibet Plateau Research Institute, and the salinity gradient of the salt lake was 0.14~118.07 g/L. This data is the result of amplification sequencing. Concentrate the lake water to 0.22 at 0.6 atm filtration pressure μ The 16S rRNA gene fragment amplification primers were 515F (5 '- GTGCCAAGCCGCGGTAA-3') and 909r (5 '- GGACTACHVGGGTWTCTAAT-3'). The Illumina MiSeq PE250 sequencer was used for end-to-end sequencing. The original data was analyzed by Mothur software. The sequence was compared with the Silva128 database and divided into operation classification units (OTUs) with 97% homology. This data can be used to analyze the microbial diversity of lakes in the Qinghai Tibet Plateau.

File naming and required software

Data reading method: offline data can be opened by text program (such as Notepad), and the main content is sequencing base quality.
OTU data is stored in excel format. The name of the file is "Microbial Distribution in Qinghai Tibet Plateau Lakes", which can be opened and read by Office and other office software

Data Citations Data citation guideline What's data citation?

Cite as:

Kong, W. (2022). Microbial data set of Qinghai Tibet Plateau Lakes (2015). A Big Earth Data Platform for Three Poles, DOI: 10.1093/femsec/fiz190. (Download the reference： RIS | Bibtex )

Related Literatures:

1. Ji, M. K., Kong, W. D., Yue, L. Y., Wang, J. B., Deng, Y., & Zhu, L. P. (2019). Salinity reduces bacterial diversity, but increases network complexity in Tibetan Plateau lakes. Fems Microbiology Ecology, 95(12), fiz190.( View Details | Bibtex)

Using this data, the data citation is required to be referenced and the related literatures are suggested to be cited.

Support Program

CASEarth:Big Earth Data for Three Poles（grant No. XDA19070000） (No:XDA19000000)

Copyright & License

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License： This work is licensed under an Attribution 4.0 International (CC BY 4.0)