The dataset contains microbial amplicon sequencing data from a total of 269 ice samples collected from 15 glaciers on the Tibetan Plateau from November 2016 to August 2020, including 24K Glacier (24K), Dongkemadi Glacier (DKMD), Dunde Glacier (DD), Jiemayangzong Glacier (JMYZ), Kuoqionggangri Glacier (KQGR), Laigu Glacier (LG), Palung 4 Glacier (PL4), Qiangtang 1 Glacier (QT), Qiangyong Glacier (QY), Quma Glacier (QM), Tanggula Glacier (TGL), Xiagangjiang Glacier (XGJ), Yala Glacier (YA), Zepugou Glacier (ZPG), ZhufengDongrongbu Glacier (ZF). The sampling areas ranged in latitude and longitude from 28.020°N to 38.100°N and 86.28°E to 95.651°E. The 16s rRNA gene was amplified by polymerase chain reaction (PCR) using 515F/907R (or 515F/806R) primers and sequenced with the Illumina Hiseq2500 sequencing platform to obtain raw data. The selected primer sequences were "515F_GTGYCAGCMGCCGCGGTAA; 907R_CCGTCAATTCMTTTRAGTTT" "515F_GTGCCAGCMGCCGCGG; 806R_ GGACTACHVGGGTWTCTAAT". The uploaded data include: sample number, sample description, sampling time, latitude and longitude coordinates, sample type, sequencing target, sequencing fragment, sequencing primer, sequencing platform, data format and other basic information. The sequencing data are stored in sequence file data format forward *.1.fq.gz and reverse *.2.fq.gz compressed files.
LIU Yongqin
This data includes the soil microbial composition data in permafrost of different ages in Barrow area of the Arctic. It can be used to explore the response of soil microorganisms to the thawing in permafrost of different ages. This data is generated by high through-put sequencing using the earth microbiome project primers are 515f – 806r. The region amplified is the V4 hypervariable region, and the sequencing platform is Illumina hiseq PE250; This data is used in the articles published in cryosphere, Permafrost thawing exhibits a greater influence on bacterial richness and community structure than permafrost age in Arctic permafrost soils. The Cryosphere, 2020, 14, 3907–3916, https://doi.org/10.5194/tc-14-3907-2020https://doi.org/10.5194/tc-14-3907-2020 . This data can also be used for the comparative analysis of soil microorganisms across the three poles.
KONG Weidong
The glacial bacterial resource database of the Tibetan Plateau provides the bacterial 16S ribosomal RNA gene sequences of several glaciers, which are seven glaciers of the Tibetan Plateau separated by an experimental group led by Yongqin Liu during 2010 to 2018 (East Rongbuk Glacier of Mt. Qomolangma, Tianshan Glacier No.1, Guliya Glacier, Laohugou Glacier, Muztagh Ata Glacier, Qiyi Glacier and Yuzhufeng Glacier), the Malan Glacier separated by Shurong Xiang and the Puruogangri Glacier separated by Xinfang Zhang. After the glacier samples were collected, they were taken to the Ecological Laboratory of the Institute of Tibetan Plateau Research of the Chinese Academy of Sciences in Beijing and the National Cryosphere Laboratory in Lanzhou. After applying the spread plate method, the samples were cultured at different temperatures (4-25 °C) for 20 days to 90 days, and single colonies were picked out for purification. After the DNA was extracted from the isolated bacteria, the 16S ribosomal RNA gene fragment was amplified with 27F/1492R primer and sequenced using the Sanger method. The 16S ribosomal RNA gene sequence was compared with the RDP database using the "Classifier" software and identified as level one when the reliability exceeded 80%. These data contain the 16S ribosomal RNA gene fragment sequence and glacier sources of each sequence. Compared with sequences based on high-throughput sequencing, these data have a longer sequence and more accurate classification and can better serve in glacier microbiology research.
JI Mukan
The data set of prokaryotic microorganism distribution in the snow and ice of the Arctic Antarctic and the Tibetan Plateau provides the bacterial 16S ribosomal RNA gene sequence collected by the experimental group led by Yongqin Liu from the NCBI database during 2010 to 2018. The keywords for NCBI database search are Antarctic, Arctic Tibetan, and Glacier. The collected sequences were calculated using the DOTOUR software to obtain the similarities between sequences, the sequences with similarities above 97% were clustered into one OTU, and the OTU representative sequence was defined. The OTU representative sequence was compared with the RDP database by the "Classifier" software and was identified as level one when the reliability exceeded 80%. After acquiring the sequence, the GPS coordinates of the sample were obtained by reading the sample information in the sequence file. These data contain the sequence of 16S ribosomal RNA gene fragments for each sequence, evolutionary classification, and sample GPS coordinates. Compared with sequences based on high-throughput sequencing, these data have a longer sequence and more accurate classification. It is significant for comparing the evolutionary information of three-pole microorganisms and understanding the evolution of psychrophilic microorganisms.
JI Mukan
The Antarctic and Arctic bacterial distribution data set provides distribution characteristics of bacteria in the Arctic and Antarctic. The collection period of the samples was from December 13,2005, to December 8,2006; 52 samples were obtained from 3 Arctic regions (Spitsbergen Slijeringa, Spitsbergen Vestpynten, and Alexandra Fjord_Highlands), and 171 samples were obtained from 5 Antarctic regions (the Mitchell Peninsula, Casey station main Power house, Robinsons Ridge, Herring Island, and Browning Peninsula). The soil surface samples were stored in liquid nitrogen after collection, shipped to a Sydney laboratory, and extracted using the FastPrep DNA kit. The extracted DNA samples were processed by 27F (5'-GAGTTTGATCNTGGCTCA-3' and 519R (5'-GTNTTACNGCGGCKGCTG-3') to amplify the 16S rRNA gene fragments. The amplified fragments were sequenced by the 454 method, and the raw data were analyzed by Mothur software. First, the sequences with poor sequencing quality were removed, the sequences were then sorted, and the chimera sequences were removed. The similarities between the sequences were calculated, the sequences with similarities above 97% were clustered into one OTU, and the OTU representative sequence was defined. By comparison with the Silva database, the OTU sequences with reliabilities greater than 80% were identified as level one. This data system compared the diversity of microorganisms in the eastern Antarctic with that in the Arctic and is of great significance for the study of the distributions of microorganisms in the Antarctic and Arctic.
JI Mukan
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