In order to describe the distribution pattern of genetic diversity of important rodents in the Qinghai Tibet Plateau, clarify their related genetic background, and establish the corresponding genetic resource bank. In 2021, this sub project (2019QZKK05010410) focused on the investigation of plateau pika in Haixi Mongolian and Tibetan Autonomous Prefecture, Golog Prefecture and Hainan prefecture, Qinghai. A total of 200 plateau pika samples were collected, and the solid samples were spleen and lung tissues. This data set contains a sample information table and habitat photos, work photos and work videos. The sample information table contains basic sample information such as species, gender, detailed sampling place, altitude, sample type, collection time, collector and storage method, which are stored in the form of Excel.
QU Jiapeng
In order to describe the distribution pattern of genetic diversity of pika germplasm resources in Qinghai Tibet Plateau, clarify its related genetic background, and establish the corresponding genetic resource bank. In 2021, this sub project (2019QZKK05010209) focused on Qinghai Province (Haixi Mongolian and Tibetan Autonomous Prefecture, Golmud City, Kunlun mountain pass; Haixi Mongolian and Tibetan Autonomous Prefecture, Dulan County, Xiangride Town, Gouli township; Golog Tibetan Autonomous Prefecture, Maduo County; Golog Tibetan Autonomous Prefecture, Maqin County, Dawu town; Huangnan Tibetan Autonomous Prefecture, Zeku County; Hainan Tibetan Autonomous Prefecture, Guinan County, Taxiu township) 93 plateau pika germplasm resources were collected at different altitudes, and the solid samples included blood or tissue, feces and so on. This data set contains 1 sample information table. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel.
ZHANG Liangzhi
In order to collect the special germplasm resources of Qinghai Tibet Plateau and excavate the molecular markers affecting the special germplasm resources, individual sheep with excellent ectopic spots were selected for marker assisted selection, propagation and generation breeding according to the genetic marker information, so as to cultivate the families of special germplasm resources. In 2021, this sub project (2019QZKK05010704) widely collected samples of Qinghai Tibetan sheep and Qinghai fine wool sheep in Haibei, Qinghai, and continued to establish and expand the first and second core groups in Ledu agricultural experimental station and Qinghai Sanjiaocheng sheep breeding farm. This data set contains the basic information of 1050 tissue samples, including variety, collection place, collection time, gender, tissue type, preservation method, etc.
ZHAO Kai
This sub project (2019qzkk05010411) focuses on the population investigation and monitoring of wild yak, Tibetan antelope and brown bear, carries out systematic and continuous field investigation, finds out their geographical distribution, population number and population structure, and carries out appropriate habitat assessment. The distribution area and potential distribution area are selected and divided into multiple sub areas. Stratified random sampling is adopted according to habitat type, climate, altitude and other factors, and the population counting method, variable distance spline method or fixed width spline method are used for investigation respectively. At the same time, in view of the extremely low encounter rate of brown bears, combined with the interview method. The investigation area mainly includes the distribution areas and potential distribution areas of the three animals in Yushu Tibetan Autonomous Prefecture, Haixi Mongolian Tibetan Autonomous Prefecture, Haibei Tibetan Autonomous Prefecture and other prefectures and counties in Qinghai Province, in order to master the distribution and five-year population dynamics of wild yak, Tibetan antelope and brown bear in Qinghai Province. This data set contains photos of yaks, Tibetan antelopes and brown bears investigated in Qinghai in 2021.
CHEN Zhenning
This sub project (2019QZKK05010217) plans to select Qinghai sand lizard, an exothermic vertebrate that is very sensitive to environmental changes, as the representative. Through field investigation, we will compare the differences in morphology, physiology and life history of Qinghai sand lizard populations at different altitudes in the Qinghai Tibet Plateau and adjacent areas, and analyze the response and adaptation characteristics of Qinghai sand lizard to environmental changes in the Qinghai Tibet Plateau, Combined with species distribution model (SDM) and mechanism model, this paper predicts the threatened degree of Qinghai sand lizard in the future, and puts forward the Protection Countermeasures of Qinghai sand lizard diversity on the Qinghai Tibet Plateau under the background of climate warming, so as to provide a theoretical basis for the protection of Reptile Diversity on the Qinghai Tibet Plateau under the background of environmental change. This data includes ecological photos and habitat photos of Qinghai sand lizard in Gonghe County, Haiyan County and Maduo County of Qinghai Province.
DU Weiguo
In order to determine the distribution points and habitat types of plateau forest frog along the latitudinal gradient in Qinghai, in 2021, a total of 8 distribution points of plateau forest frog were collected in Minhe County of Haidong, Qinghai, Gonghe County of Hainan Tibetan Autonomous Prefecture and Maqin County of Golog Prefecture, covering an altitude of 2000-3800m. This dataset contains 1 coordinate information table and 57 habitat photos. The coordinate information table contains information such as number, recording date, time, weather, coordinate longitude and latitude, altitude sample, habitat type and photo number of representative habitat, which are stored in the form of Excel. Photos are stored in JPG format. In order to reveal the impact of climate change on the diversity of plateau forest frogs on the Qinghai Tibet Plateau in the future, the thermal safety margin of different geographical populations of plateau forest frogs was calculated through the data of thermophysiological indexes and environmental effective temperature, and the threat of climate warming of different geographical populations of plateau forest frogs was evaluated. This data set includes field activity body temperature, resting metabolic rate, ambient temperature and morphological data of four altitude populations of plateau forest frog, which supplements the selected body temperature, tolerance temperature and temperature correction data of 2000 m altitude populations. The data is stored in Excel format. In order to study the differences of genetic diversity of plateau forest frog at different altitudes, 100 samples of plateau forest frog collected from four altitudes (2000 m, 2600 m, 3200 m and 3800 m) of Qinghai Tibet Plateau were sequenced and analyzed based on the sequences of four mitochondrial genes (12S rRNA, 16S rRNA, coi and cytb), so as to provide scientific basis for the protection of this species. This data includes 12S rRNA, 16S rRNA The sequence data obtained from the sequencing of COI and cytb genes supplement the sequence data of four genes of the population at an altitude of 2000m. The data is stored in FASTA format
ZHANG Yongpu
In order to describe the effects of environmental changes and human disturbance on the temporal and spatial distribution of small mammals in the Qinghai Tibet Plateau, statistical models and molecular techniques were used to identify the species of small mammals with different altitude gradients. In 2021, 105 small mammal resources such as plateau pika, Meriones meridionalis, three toed jerboa, five toed jerboa and Qinghai squirrel were collected in 8 areas of Xihai Town, Gangcha county and Delingha city of Qinghai Province. The solid samples include animal solid samples and muscles, heart, liver, spleen, lung, kidney, pancreas, bladder, testis and ovary. This data set contains one specimen information table, one tissue sample information table and one photo corresponding to each specimen. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel. Photos, stored in JPG format.
HOU Xiang
In November 2020, we made a collection in Qinghai Tibet Plateau were collected by net and electric capture methods, and the sampling area included the main water systems in Qinghai Province. A total of 30 sampling points were collected, and 685 fish specimens were collected in 12 points, including Schizothorax of loach.This work is a part of the project of “Building Methods for Detection of Aquatic Organisms in the Lake System of the Qinghai-Tibet Plateau”, using traditional fish survey data to generate a list of species in the lake system, which will then be used to combine multiple lakes in the plateau. High-throughput molecular data acquired from the system's environmental water samples and tested for visual parameters (lake size, isolation, geographic location, and spectral characteristics) that can be used to predict aquatic biodiversity.
LIU Shuwei
From April 2020 to August 2020, sub project 3 collected 51 ear tissue samples of Qinghai fine wool sheep distributed in Haiyan County, Haixi Mongolian and Tibetan Autonomous Prefecture, Qinghai Province, 50 blood samples of Oula sheep in Tongde County, Hainan Tibetan Autonomous Prefecture, 50 blood samples of yak in Tongde County, Hainan Tibetan Autonomous Prefecture, 60 blood samples of Haidong donkey in Datong Hui and Tu Autonomous County, Xining City, and tissue samples A total of 211 copies. At the same time, the information of body length, body height, weight, age and gender, as well as the data of economic traits such as litter size, wool fineness and wool length were recorded. The individual photos were taken, and the information of feeding mode and epidemic situation were obtained through questionnaire survey.
TIAN Fei
1) data content: including the morphological and reproductive life history data of four lizards, i.e. Phrynocephalus vlangalii, Phrynocephalus przewalskii, Eremias argus and Eremias multiocellata, and the physiological and ecological data of indoor and outdoor activity temperature, selection temperature, tolerant high temperature and tolerant low temperature, which is helpful to understand and analyze the physiological and ecological characteristics of typical lizards. 2) data source and processing method: Based on the indoor and outdoor experiments of typical lizards on the Qinghai Tibet Plateau and pan third pole from 2013 to 2019, the data of physiological and ecological indicators of lizards in the field and the data of reproductive life history of pregnant lizards were recorded. 3) data quality description: the lizard indoor and outdoor data collectors are all graduate students, who have been trained strictly to ensure the quality of the collected data. 4) data application achievements and prospects: Taking the typical lizards in the Qinghai Tibet Plateau and surrounding areas as the object, focusing on the impact of climate change on the thermal regulation behavior and reproductive life history of lizards, obtaining the physiological and ecological change characteristics of lizards under the climate change conditions is helpful to simulate and analyze the response trend of lizards distribution and population change under the climate warming environment.
ZENG Zhigao
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