This data includes bacterial 16S ribosomal RNA gene sequence data from 25 lakes in the middle of the Qinghai Tibet Plateau. The sample was collected from July to August 2015, and the surface water was sampled three times with a 2.5 liter sampler. The samples were immediately taken back to the Ecological Laboratory of the Beijing Qinghai Tibet Plateau Research Institute, and the salinity gradient of the salt lake was 0.14~118.07 g/L. This data is the result of amplification sequencing. Concentrate the lake water to 0.22 at 0.6 atm filtration pressure μ The 16S rRNA gene fragment amplification primers were 515F (5 '- GTGCCAAGCCGCGGTAA-3') and 909r (5 '- GGACTACHVGGGTWTCTAAT-3'). The Illumina MiSeq PE250 sequencer was used for end-to-end sequencing. The original data was analyzed by Mothur software. The sequence was compared with the Silva128 database and divided into operation classification units (OTUs) with 97% homology. This data can be used to analyze the microbial diversity of lakes in the Qinghai Tibet Plateau.
2022-10-14
This data includes the distribution data of soil bacteria in Namco region of the Qinghai Tibet Plateau, which can be used to explore the seasonal impact of fencing and grazing on soil microorganisms in Namco region. The sample was collected from May to September 2015, and the soil samples were stored in ice bags and transported back to the Ecological Laboratory of Beijing Institute of Qinghai Tibet Plateau Research; This data is the result of amplification sequencing, using MoBio Powersoil ™ Soil DNA was extracted with DNA isolation kit, and the primers were 515F (5 '- GTGCCAAGCGCCGGTAA-3') and 806R (5'GGACTACNVGGGTWTCTAAT-3 '). The amplified fragments were sequenced by Illumina Miseq PE250. The original data is analyzed by Qiime software, and then the similarity between sequences is calculated, and the sequences with a similarity of more than 97% are clustered into an OTU. The Greengenes reference library is used for sequence alignment to remove the sequence that only appears once in the database. The soil moisture content and soil temperature were measured by a soil hygrometer, and the soil pH was measured by a pH meter (Sartorius PB-10, Germany). The soil nitrate nitrogen (NO3 −) and ammonium nitrogen (NH4+) concentrations were extracted with 2 M KCl (soil/solution, 1:5), and analyzed with a Smartchem200 discrete automatic analyzer. This data set is of great significance to the study of soil microbial diversity in arid and semi-arid grasslands.
2022-10-14
Data on soil bacterial diversity of grassland in Qinghai Tibet Plateau. The samples were collected from July to August 2017, including 120 samples of alpine meadow, typical grassland and desert grassland. The soil surface samples were collected and stored in ice bags, and then transported back to the ecological laboratory of the Beijing Qinghai Tibet Plateau Research Institute. The soil DNA was extracted by MO BIO PowerSoil DNA kit. The 16S rRNA gene fragment amplification primers were 515F (5 '- GTGCCAAGCCGGTAA-3') and 806R (5 ´ GGACTACNVGGGTWTCTAAT-3 ´). The amplified fragments were sequenced by Illumina Miseq PE250. The original data is analyzed by Qiime software, and the sequence classification is based on the Silva128 database. Sequences with a similarity of more than 97% are clustered into an operation classification unit (OTU). This data systematically compares the bacterial diversity of soil microorganisms in the Qinghai Tibet Plateau transect, which is of great significance to the study of the distribution of microorganisms in the Qinghai Tibet Plateau.
2022-10-14
The data set contains the rare animal survey data for the Sanjiangyuan area from 2016 to 2017, including the latitude and longitude of the survey site, the length of the sample line, animal discovery time, animal names, quantity, location of the occurrence, type of habitat, affiliated families, etc.
2021-04-18
This data includes the soil microbial composition data in permafrost of different ages in Barrow area of the Arctic. It can be used to explore the response of soil microorganisms to the thawing in permafrost of different ages. This data is generated by high through-put sequencing using the earth microbiome project primers are 515f – 806r. The region amplified is the V4 hypervariable region, and the sequencing platform is Illumina hiseq PE250; This data is used in the articles published in cryosphere, Permafrost thawing exhibits a greater influence on bacterial richness and community structure than permafrost age in Arctic permafrost soils. The Cryosphere, 2020, 14, 3907–3916, https://doi.org/10.5194/tc-14-3907-2020https://doi.org/10.5194/tc-14-3907-2020 . This data can also be used for the comparative analysis of soil microorganisms across the three poles.
2021-04-09
1. The grassland animal husbandry production and management policies in the study area from 1954 to 2012 mainly include: 1) the time series of the formation and evolution of various policies; 2) the key policies related to herdsman's livestock activities and grassland management and utilization. 2. Residents' perception and response to pastoral socio-economic development policies, grassland management systems, ecological compensation policies, ecological restoration projects, and ecological environment status quo.
2020-04-12
Taking Landsat series data as the main data source, including KH in 1965 (only including Gurinai and Guaizi Lake), MSS in 1975, TM in 1990, 1995, 2006 and 2010, and ETM in 2000. Before information extraction, remote sensing images are preprocessed by image synthesis, mosaic, fusion, geometric correction and image enhancement. In the process of correction, ETM + image in 2000 is corrected by 1:100000 topographic map and used as reference image. The 4, 3 and 2 band standard pseudocolor synthesis scheme is selected for image synthesis; during correction, 7 × 8 control points are evenly selected on each image, and the average positioning error is less than 1 pixel, that is, the ground distance is less than 30m. In other years, the datum image of 2000 is used as the reference image for image registration, so that the pixels with the same name on different images have the same geographical coordinates. After correction and registration, the whole image maintains the 30 m spatial resolution of TM. Through field correction, the accuracy of qualitative analysis can be ensured to be over 95%.
2020-02-21
The data set was obtained from the background survey of wildlife diversity in Three River Source National Park by Northwest Institute of Plateau Biology, Chinese Academy of Sciences. The time range of the data set is 2017, and the survey area is Three River Source National Park. The survey species include a variety of rare wildlife such as Equus kiang, Canis lupus, Vulpes vulpes, Cervus elaphus, Accipiter nisus, Phoenicurus erythrogastrus, Prionailurus bengalensis, Buteo hemilasius, Procapra picticaudata, Tetraogallus tibetanus, Perdix hodgsoniae, Falco cherrug, etc.
2019-05-28
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