The data include raw sequencing result of plant DNA in surface sediments of 33 lakes in the Qinghai-Tibetan Plateau and arid northwestern China. We used PowerMax Soil Kit of Qiagen company in Germany to extract DNA, then used universal plant primer g-h (Taberlet et et al., 2007) to amplify P6 loop of chloroplast trnL (UAA) intron in the sample. The PCR products were then sent to Fasteris company in Switzerland for the next-generation paired-end sequencing. The sequencing instrument is Illumina Nextseq 550. The data quality score (Q30) is 81.97.

File naming and required software

The data is fastq file. It is recommended to use obitools and other bioinformatic tools to read and analyze the data. Please refer to https://datadryad.org/stash/dataset/doi :10.5061/dryad.k6djh9w4r.

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Cite as:

Liu, X., Jia, W. (2021). Data of plant DNA in surface sediments of 33 lakes in Qinghai-Tibetan Plateau and arid northwestern China. A Big Earth Data Platform for Three Poles, DOI: 10.5061/dryad.k6djh9w4r. (Download the reference： RIS | Bibtex )

Related Literatures:

1. Stoof-Leichsenring KR, Liu S, Jia W, Li K, Pestryakova LA, Mischke S, Cao X, Liu X, Ni J, Neuhaus S, Herzschuh U (2020) Plant diversity in sedimentary DNA obtained from high-latitude (Siberia) and high-elevation lakes (China). Biodiversity Data Journal 8: e57089. https://doi.org/10.3897/BDJ.8.e57089( View Details | Bibtex)

2. Jia, W. (2020). Modern-process studies of plant DNA in lake sediments, Qinghai-Tibetan Plateau and arid northwestern China. Capital Normal University, Beijing, China.( View Details | Bibtex)

Using this data, the data citation is required to be referenced and the related literatures are suggested to be cited.

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