This data includes the soil microbial composition data in permafrost of different ages in Barrow area of the Arctic. It can be used to explore the response of soil microorganisms to the thawing in permafrost of different ages. This data is generated by high through-put sequencing using the earth microbiome project primers are 515f – 806r. The region amplified is the V4 hypervariable region, and the sequencing platform is Illumina hiseq PE250; This data is used in the articles published in cryosphere, Permafrost thawing exhibits a greater influence on bacterial richness and community structure than permafrost age in Arctic permafrost soils. The Cryosphere, 2020, 14, 3907–3916, https://doi.org/10.5194/tc-14-3907-2020https://doi.org/10.5194/tc-14-3907-2020 . This data can also be used for the comparative analysis of soil microorganisms across the three poles.

File naming and required software

The dataset contains two file types: sequence data (.fastq) and OTU table (.xlsx).
File name: the name of the sequence data file is: permafrost age + thaw status+replication ID.read.fq , permafrost age is the age of the perfofrost, including young (< 50 years old), medium (< 300 years old), old (< 3000 years old) and ancient (3000 – 5000 years old); thaw status is the permafrost state, including: permanently frozen, transition and active; replication ID includes 1-4; read is the sequencing direction, including R1 (forward) and R2 (reverse)
Data reading method: the sequence data can be opened by text program (such as Notepad), and the main content is sequencing base quality; the processed OTU table can be opened by MS Excel, and the subject content is OTU table based on 97% sequence identity and species classification information.

Data Citations Data citation guideline What's data citation?

Cite as:

Kong, W. (2021). Permafrost soil bacteria in Barrow Peninsula, Arctic (2015). A Big Earth Data Platform for Three Poles, DOI: 10.11888/Soil.tpdc.271248. CSTR: 18406.11.Soil.tpdc.271248. (Download the reference： RIS | Bibtex )

Related Literatures:

1. Ji, M., Kong, W., Liang, C., Zhou, T., Jia, H., and Dong, X. (2020). Permafrost thawing exhibits a greater influence on bacterial richness and community structure than permafrost age in Arctic permafrost soils. The Cryosphere, 14, 3907–3916, https://doi.org/10.5194/tc-14-3907-2020.( View Details | Bibtex)

Using this data, the data citation is required to be referenced and the related literatures are suggested to be cited.

Support Program

CASEarth:Big Earth Data for Three Poles（grant No. XDA19070000） (No:XDA19000000)

Copyright & License

To respect the intellectual property rights, protect the rights of data authors, expand services of the data center, and evaluate the application potential of data, data users should clearly indicate the source of the data and the author of the data in the research results generated by using the data (including published papers, articles, data products, and unpublished research reports, data products and other results). For re-posting (second or multiple releases) data, the author must also indicate the source of the original data.

License： This work is licensed under an Attribution 4.0 International (CC BY 4.0)