Microbial amplicon sequencing dataset for glacial ice and snow on the Tibetan Plateau (V1.0) (2016-2020)- A Big Earth Data Platform for Three Poles

The dataset contains microbial amplicon sequencing data from a total of 269 ice samples collected from 15 glaciers on the Tibetan Plateau from November 2016 to August 2020, including 24K Glacier (24K), Dongkemadi Glacier (DKMD), Dunde Glacier (DD), Jiemayangzong Glacier (JMYZ), Kuoqionggangri Glacier (KQGR), Laigu Glacier (LG), Palung 4 Glacier (PL4), Qiangtang 1 Glacier (QT), Qiangyong Glacier (QY), Quma Glacier (QM), Tanggula Glacier (TGL), Xiagangjiang Glacier (XGJ), Yala Glacier (YA), Zepugou Glacier (ZPG), ZhufengDongrongbu Glacier (ZF). The sampling areas ranged in latitude and longitude from 28.020°N to 38.100°N and 86.28°E to 95.651°E.

The 16s rRNA gene was amplified by polymerase chain reaction (PCR) using 515F/907R (or 515F/806R) primers and sequenced with the Illumina Hiseq2500 sequencing platform to obtain raw data. The selected primer sequences were "515F_GTGYCAGCMGCCGCGGTAA; 907R_CCGTCAATTCMTTTRAGTTT" "515F_GTGCCAGCMGCCGCGG; 806R_ GGACTACHVGGGTWTCTAAT". The uploaded data include: sample number, sample description, sampling time, latitude and longitude coordinates, sample type, sequencing target, sequencing fragment, sequencing primer, sequencing platform, data format and other basic information. The sequencing data are stored in sequence file data format forward *.1.fq.gz and reverse *.2.fq.gz compressed files.

File naming and required software

File naming: The sample number naming rule is "yymm g type num-add", where yy represents the year, mm represents the month, g represents the glacier abbreviation, type is the sample type (I: ice ice; SN: snow snow), num represents the sample serial number, and add is additional information. For example, 190624KSN1 would represent a 24K glacier surface snow sample collected in June 2019. Sequencing data are organized by different glaciers (glacier abbreviations).
Data readout: tables can be opened directly using Excel tools. Amplicon sequencing data (fastq files) can be opened by sequencing data analysis software.

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Cite as:

Liu, Y. (2022). Microbial amplicon sequencing dataset for glacial ice and snow on the Tibetan Plateau (V1.0) (2016-2020). A Big Earth Data Platform for Three Poles, DOI: 10.11888/HumanNat.tpdc.272502. CSTR: 18406.11.HumanNat.tpdc.272502. (Download the reference： RIS | Bibtex )

Using this data, the data citation is required to be referenced and the related literatures are suggested to be cited.

Support Program

Second Tibetan Plateau Scientific Expedition Program

Copyright & License

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License： This work is licensed under an Attribution 4.0 International (CC BY 4.0)