This data includes the distribution data of soil bacteria in Namco region of the Qinghai Tibet Plateau, which can be used to explore the seasonal impact of fencing and grazing on soil microorganisms in Namco region. The sample was collected from May to September 2015, and the soil samples were stored in ice bags and transported back to the Ecological Laboratory of Beijing Institute of Qinghai Tibet Plateau Research; This data is the result of amplification sequencing, using MoBio Powersoil ™ Soil DNA was extracted with DNA isolation kit, and the primers were 515F (5 '- GTGCCAAGCGCCGGTAA-3') and 806R (5'GGACTACNVGGGTWTCTAAT-3 '). The amplified fragments were sequenced by Illumina Miseq PE250. The original data is analyzed by Qiime software, and then the similarity between sequences is calculated, and the sequences with a similarity of more than 97% are clustered into an OTU. The Greengenes reference library is used for sequence alignment to remove the sequence that only appears once in the database. The soil moisture content and soil temperature were measured by a soil hygrometer, and the soil pH was measured by a pH meter (Sartorius PB-10, Germany). The soil nitrate nitrogen (NO3 −) and ammonium nitrogen (NH4+) concentrations were extracted with 2 M KCl (soil/solution, 1:5), and analyzed with a Smartchem200 discrete automatic analyzer. This data set is of great significance to the study of soil microbial diversity in arid and semi-arid grasslands.

File naming and required software

File name: N+month, read is the sequencing direction, including R1 (forward) and R2 (reverse)
Data reading method: offline data can be opened by text program (such as Notepad), and the main content is sequencing base quality.
The processed OTU table can be opened with office software, and the subject content is the OTU table and species classification information based on 97% sequence identity.

Data Citations Data citation guideline What's data citation?

Cite as:

Kong, W. (2022). Data set of soil microbial diversity in Namco, Qinghai Tibet Plateau (2015). A Big Earth Data Platform for Three Poles, DOI: 10.1002/ldr.3626. (Download the reference： RIS | Bibtex )

Related Literatures:

1. Fan, D. D., Kong, W. D., Wang, F., Yue, L. Y., & Li, X. Z. (2020). Fencing decreases microbial diversity but increases abundance in grassland soils on the Tibetan Plateau. Land Degradation & Development, 31(17), 2577-2590.( View Details | Bibtex)

Using this data, the data citation is required to be referenced and the related literatures are suggested to be cited.

Support Program

CASEarth:Big Earth Data for Three Poles（grant No. XDA19070000） (No:XDA19000000)

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